Candiotto F B, Freitas-Júnior A C V, Neri R C A, Bezerra R S, Rodrigues R V, Sampaio L A, Tesser M B
Programa de Pós-graduação em Aquicultura, Instituto de Oceanografia, Universidade Federal do Rio Grande, Rio Grande, RS, Brazil.
Departamento de Biologia Molecular, Centro de Ciências Exatas e da Natureza, Universidade Federal da Paraíba, João Pessoa, PB, Brazil.
Braz J Biol. 2018 May;78(2):281-288. doi: 10.1590/1519-6984.06616. Epub 2017 Aug 17.
Knowledge of specific enzyme activity, along with animal habits and digestive capacity is essential in formulating an appropriate diet for any species. In this study, we evaluated and characterized the activity of digestive enzymes present in the liver, intestine, and stomach of Paralichthys orbignyanus. The effects of pH and temperature on enzyme activity were also evaluated via the use of specific substrates. The use of specific substrates and inhibitors showed strong evidence of the presence of trypsin (BApNA= 0.51 ± 0.2 mU mg-1), chimotrypsin (SApNA= 2.62 ± 1.8 mU mg-1), and aminopeptidases (Leu-p-Nan =0.9709 ± 0.83 mU mg-1) in the intestine. Optimum pH for the activity of trypsin, chemotrypsin, leucino aminopeptidase, amilase, and pepsin were 9.5, 9.0, 8.0, 7.5, and 3.5, respectively, while optimum temperatures were 50, 50, 50, 40, and 45 °C, respectively. These results provide additional information regarding the biology of Brazilian flounder and can be used as a basis for further studies regarding fish feeding physiology.
了解特定酶的活性，以及动物习性和消化能力，对于为任何物种制定合适的饮食至关重要。在本研究中，我们评估并表征了奥氏牙鲆肝脏、肠道和胃中存在的消化酶活性。还通过使用特定底物评估了pH值和温度对酶活性的影响。使用特定底物和抑制剂有力地证明了肠道中存在胰蛋白酶（BApNA = 0.51 ± 0.2 mU mg-1）、糜蛋白酶（SApNA = 2.62 ± 1.8 mU mg-1）和氨肽酶（Leu-p-Nan = 0.9709 ± 0.83 mU mg-1）。胰蛋白酶、糜蛋白酶、亮氨酰氨肽酶、淀粉酶和胃蛋白酶活性的最佳pH值分别为9.5、9.0、8.0、7.5和3.5，而最佳温度分别为50、50、50、40和45°C。这些结果提供了有关巴西牙鲆生物学的更多信息，可作为进一步研究鱼类摄食生理学的基础。
