Martín Muñoz P, Anel-López L, Ortiz-Rodríguez J M, Álvarez M, de Paz P, Balao da Silva C, Rodríguez Martinez H, Gil M C, Anel L, Peña F J, Ortega Ferrusola C
Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.
Reproduction and Obstetrics Department of Animal Medicine and Surgery, University of León, León, Spain.
Reprod Domest Anim. 2018 Feb;53(1):54-67. doi: 10.1111/rda.13052. Epub 2017 Aug 17.
Oxidative stress is a major factor explaining sperm dysfunction of spermatozoa surviving freezing and thawing and is also considered a major inducer of a special form of apoptosis, visible after thawing, in cryopreserved spermatozoa. To obtain further insights into the link between oxidative stress and the induction of apoptotic changes, stallion spermatozoa were induced to oxidative stress through redox cycling after exposure to 2-methyl-1,4-naphthoquinone (menadione), or hydroxyl radical formation after FeSO exposure. Either exposure induced significant increases (p < 0.05) in two markers of lipid peroxidation: 8-iso-PGF and 4-hydroxynonenal (4-HNE). While both treatments induced changes indicative of spermptosis (caspase-3 activation and decreased mitochondrial membrane potential) (p < 0.01), menadione induced sperm necrosis and a dramatic reduction in motility and thiol content in stallion spermatozoa. Thus, we provided evidence that oxidative stress underlies spermptosis, and thiol content is a key factor for stallion sperm function.
氧化应激是解释冻融后存活精子功能障碍的主要因素，也是冻存精子解冻后可见的一种特殊形式细胞凋亡的主要诱导因素。为了进一步深入了解氧化应激与凋亡变化诱导之间的联系，马精子在暴露于2-甲基-1,4-萘醌（甲萘醌）后通过氧化还原循环被诱导产生氧化应激，或在暴露于硫酸亚铁后产生羟基自由基。两种暴露均导致脂质过氧化的两个标志物8-异前列腺素F2α（8-iso-PGF）和4-羟基壬烯醛（4-HNE）显著增加（p < 0.05）。虽然两种处理均诱导了表明精子凋亡的变化（半胱天冬酶-3激活和线粒体膜电位降低）（p < 0.01），但甲萘醌诱导了马精子的坏死以及活力和硫醇含量的显著降低。因此，我们提供了证据表明氧化应激是精子凋亡的基础，并且硫醇含量是马精子功能的关键因素。
