中空 ZnO 纳米球通过促进体内 CD4 和 CD8 T 细胞群体增强抗癌免疫。

Hollow ZnO Nanospheres Enhance Anticancer Immunity by Promoting CD4 and CD8 T Cell Populations In Vivo.

机构信息

Health Research Institute, Department of Life Science and Biotechnology, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan.

Biomedical Research Institute, Department of Life Science and Biotechnology, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan.

出版信息

Small. 2017 Oct;13(38). doi: 10.1002/smll.201701816. Epub 2017 Aug 21.

DOI:10.1002/smll.201701816

PMID:28834273

Abstract

Appropriate adjuvant aiding in generating robust anticancer immunity is crucial for cancer immunotherapy. Herein, hollow ZnO (HZnO) nanospheres are synthesized by a facile method using carbon nanospheres as the template. The HZnO nanospheres significantly promote the cellular uptake of a model antigen, and cytokine secretion by antigen-presenting cells in vitro. HZnO loaded with ovalbumin and polyinosinic-polycytidylic acid (poly(I:C)) inhibits cancer growth and metastasis to inguinal lymph node in a cancer cell challenge model. Moreover, HZnO loaded with autologous cancer antigens inhibits cancer cell growth in a cancer cell re-challenge model. HZnO nanospheres significantly improve the CD4 and/or CD8 T cell population in splenocytes of mice in both cancer cell challenge model and re-challenge model. The HZnO nanospheres can be used for cancer immunotherapy as adjuvant.

摘要

适当的佐剂有助于产生强大的抗癌免疫是癌症免疫治疗的关键。在此，通过简便的方法使用碳纳米球作为模板合成了空心氧化锌 (HZnO) 纳米球。HZnO 纳米球显著促进了模型抗原的细胞摄取，以及抗原呈递细胞在体外的细胞因子分泌。负载卵清蛋白和聚肌苷酸-聚胞苷酸 (poly(I:C)) 的 HZnO 抑制了癌细胞攻击模型中腹股沟淋巴结的肿瘤生长和转移。此外，负载自体肿瘤抗原的 HZnO 抑制了癌细胞再攻击模型中的肿瘤细胞生长。HZnO 纳米球显著增加了癌细胞攻击模型和再攻击模型中小鼠脾细胞中的 CD4 和/或 CD8 T 细胞群体。HZnO 纳米球可用作癌症免疫治疗的佐剂。