Rozenfeld Julio H K, Duarte Evandro L, Oliveira Tiago R, Lamy M Teresa
Departamento de Biofísica, Escola Paulista de Medicina, Universidade Federal de São Paulo, R. Botucatu 862, São Paulo, SP, 04023-062, Brazil.
Instituto de Física, Universidade de São Paulo, R. do Matão 1371, São Paulo, SP, 05508-090, Brazil.
Biophys Rev. 2017 Oct;9(5):633-647. doi: 10.1007/s12551-017-0304-4. Epub 2017 Aug 23.
Cationic bilayers have been used as models to study membrane fusion, templates for polymerization and deposition of materials, carriers of nucleic acids and hydrophobic drugs, microbicidal agents and vaccine adjuvants. The versatility of these membranes depends on their structure. Electron spin resonance (ESR) spectroscopy is a powerful technique that employs hydrophobic spin labels to probe membrane structure and packing. The focus of this review is the extensive structural characterization of cationic membranes prepared with dioctadecyldimethylammonium bromide or diC14-amidine to illustrate how ESR spectroscopy can provide important structural information on bilayer thermotropic behavior, gel and fluid phases, phase coexistence, presence of bilayer interdigitation, membrane fusion and interactions with other biologically relevant molecules.
阳离子双层已被用作研究膜融合的模型、材料聚合和沉积的模板、核酸和疏水药物的载体、杀微生物剂和疫苗佐剂。这些膜的多功能性取决于它们的结构。电子自旋共振（ESR）光谱是一种强大的技术，它使用疏水自旋标记来探测膜的结构和堆积。本综述的重点是用二辛基二甲基溴化铵或二C14-脒制备的阳离子膜的广泛结构表征，以说明ESR光谱如何能够提供关于双层热致行为、凝胶相和流体相、相共存、双层交叉指状结构的存在、膜融合以及与其他生物相关分子相互作用的重要结构信息。
