Li Wen-Juan, Chang Lan, Liu Qiao, Ning Di, Yao Xi-Yuan, Li Yue, Ruan Wen-Juan
College of Chemistry, Key Laboratory of Advanced Energy Materials, Chemistry (Ministry of Education), Nankai University, Tianjin, 300071, P. R. China.
Chemistry. 2017 Nov 2;23(61):15498-15504. doi: 10.1002/chem.201703438. Epub 2017 Oct 10.
As novel fluorescent-sensing materials, metal-organic frameworks (MOFs) have shown great potential in environmental monitoring. However, most of the researches are limited to traditional pollutants, whereas the application of MOFs to the detection of the pollutants with more complicated structures, such as endocrine disrupting chemicals (EDCs), has rarely been explored. The difficulties faced in the sensing of EDCs include their electronic stability and the structural similarity among homologues, which could be overcome by the incorporation of enzymatic reaction. In this work, the first example of enzyme-assisted MOF-fluorescent-sensing was developed for the analysis of diethylstilbestrol (DES, a synthetic estrogen). In this system, DES is first oxidized by HRP/H O quantitatively to its quinone form, and then the quinone product is selectively captured by a stilbene based luminescent MOF to induce fluorescence response. By the tandem sensitization and filtration of enzymatic reaction and MOF adsorption, this method shows high sensitivity (DL=89 nm) and can distinguish DES from other similar-structured EDCs.
作为新型荧光传感材料，金属有机框架（MOFs）在环境监测中显示出巨大潜力。然而，大多数研究仅限于传统污染物，而MOFs在检测结构更复杂的污染物（如内分泌干扰化学物质（EDCs））方面的应用却很少被探索。检测EDCs面临的困难包括其电子稳定性以及同系物之间的结构相似性，而通过引入酶促反应可以克服这些困难。在这项工作中，开发了首个酶辅助MOF荧光传感用于分析己烯雌酚（DES，一种合成雌激素）的实例。在该体系中，DES首先被HRP/H₂O₂定量氧化为醌形式，然后醌产物被基于芪的发光MOF选择性捕获以诱导荧光响应。通过酶促反应和MOF吸附的串联敏化和筛选，该方法显示出高灵敏度（检测限=89 nM），并且能够将DES与其他结构相似的EDCs区分开来。
