Schwarz M, Appel K E, Rickart R, Kunz H W
Arch Toxicol Suppl. 1979(2):479-82. doi: 10.1007/978-3-642-67265-1_60.
Viable rat liver cells were isolated, and the velocity sedimentation characteristics of DNA centrifuged through alkaline sucrose gradients were studied following the administration in vivo of dimethylnitrosamine (DMN), phenobarbital and halothane. DMN application resulted in a strong decrease of sedimentation velocity, indicating generation of single strand breaks in DNA, whereas phenobarbital and halothane given in doses sufficient to induce liver enlargement did not alter the sedimentation behaviour of DNA from parenchymal or extraparenchymal liver cells.
分离出有活力的大鼠肝细胞，并在体内给予二甲基亚硝胺（DMN）、苯巴比妥和氟烷后，研究通过碱性蔗糖梯度离心的DNA的速度沉降特性。应用DMN导致沉降速度大幅下降，表明DNA中产生了单链断裂，而给予足以诱导肝脏肿大剂量的苯巴比妥和氟烷并未改变实质或肝外肝细胞DNA的沉降行为。
