Gesing Julia, Scheuermann Kathrin, Wagner Isabel Viola, Löffler Dennis, Friebe Daniela, Kiess Wieland, Schuster Volker, Körner Antje
Hospital for Children and Adolescents, University Leipzig, Leipzig, Saxony, Germany.
Leipzig University Medical Center (IFB) AdiposityDiseases, Leipzig, Saxony, Germany.
PLoS One. 2017 Aug 24;12(8):e0183027. doi: 10.1371/journal.pone.0183027. eCollection 2017.
Nicotinamide phosphoribosyl transferase (NAMPT) is an inflammatory adipocytokine shown to interact in immune modulation in chronic inflammatory diseases, acute respiratory distress syndrome, sepsis, cancer and obesity in adulthood. It is, however, not clear whether this association reflects a chronic elevation or acute inflammatory response. We analyzed NAMPT concentrations in distinct states of inflammation in 102 children and found consistently significantly increased NAMPT levels in subjects with acute infections. NAMPT concentrations in children with stable chronic inflammatory diseases were not significantly different, whereas in patients with acute relapse of chronic disease NAMPT was significantly higher than in children in remission or healthy controls. In states of low-grade inflammation (children with atopic disease or obesity) we did not detect alterations in NAMPT serum levels. NAMPT correlated positively with inflammatory markers such as CRP. The most predictive factor for NAMPT serum concentrations was leucocyte count and therein the neutrophil count. Furthermore, systemic circulating NAMPT levels were closely associated with NAMPT release from corresponding cultured PBMCs. In conclusion, NAMPT is selectively increased in states of acute but not chronic inflammation in children. The close relationship between systemic circulating NAMPT with leucocyte counts and release indicate that leucocytes most probably are the source of inflammation related NAMPT levels.
烟酰胺磷酸核糖转移酶（NAMPT）是一种炎症性脂肪细胞因子，在慢性炎症性疾病、急性呼吸窘迫综合征、脓毒症、癌症和成人肥胖症的免疫调节中发挥相互作用。然而，这种关联是反映慢性升高还是急性炎症反应尚不清楚。我们分析了102名儿童在不同炎症状态下的NAMPT浓度，发现急性感染患儿的NAMPT水平持续显著升高。患有稳定慢性炎症性疾病的儿童NAMPT浓度无显著差异，而慢性疾病急性复发的患者NAMPT显著高于缓解期儿童或健康对照。在低度炎症状态（患有特应性疾病或肥胖症的儿童）中，我们未检测到NAMPT血清水平的变化。NAMPT与CRP等炎症标志物呈正相关。NAMPT血清浓度的最具预测性因素是白细胞计数，尤其是中性粒细胞计数。此外，全身循环的NAMPT水平与相应培养的外周血单核细胞释放的NAMPT密切相关。总之，儿童急性而非慢性炎症状态下NAMPT选择性升高。全身循环的NAMPT与白细胞计数和释放之间的密切关系表明，白细胞很可能是炎症相关NAMPT水平的来源。
