Department of Pediatrics, Maternal and Child Health Hospital of Hubei Province (Women and Children's Hospital of Hubei Province), Wuhan, Hubei, China.
Department of Pediatrics, Wuhan No. 1 Hospital, Wuhan, Hubei, China.
Biosci Rep. 2020 Jul 31;40(7). doi: 10.1042/BSR20201267.
Neonatal septicemia is a serious infectious disease in the neonatal period. MicroRNAs (miRNAs) have been reported to participate in the inflammatory responses in neonatal sepsis. The aim of the present study was to explore the effects and molecular mechanism of miR-96-5p on regulating the inflammatory responses in neonatal sepsis. MiR-96-5p was low expressed while nicotinamide phosphoribosyltransferase (NAMPT) was high expressed in the serum of neonatal septicemia patients. The expression of miR-96-5p was decreased in LPS-induced inflammatory responses. Besides, miR-95-5p relieved LPS-induced inflammatory responses in RAW264.7 cells. NAMPT was demonstrated as a potential target of miR-96-5p, and knockdown of NAMPT reduced inflammatory in RAW264.7 cells stimulated with LPS. Moreover, overexpression of NAMPT reversed the effects of miR-96-5p on LPS-induced inflammatory responses. In addition, miR-96-5p inhibited nuclear factor (NF)-κB signaling pathway in RAW264.7 cells stimulated with LPS. MiR-96-5p alleviated inflammatory responses via targeting NAMPT and inhibiting NF-κB pathway in neonatal sepsis.
新生儿败血症是新生儿期的一种严重感染性疾病。有研究报道，微小 RNA（miRNA）参与新生儿败血症的炎症反应。本研究旨在探讨 miR-96-5p 对调节新生儿败血症炎症反应的作用及其分子机制。新生儿败血症患者血清中 miR-96-5p 表达下调，烟酰胺磷酸核糖转移酶（NAMPT）表达上调。miR-96-5p 在 LPS 诱导的炎症反应中表达下调。此外，miR-96-5p 减轻了 LPS 诱导的 RAW264.7 细胞的炎症反应。NAMPT 被证明是 miR-96-5p 的一个潜在靶点，敲低 NAMPT 可减少 LPS 刺激的 RAW264.7 细胞的炎症反应。此外，过表达 NAMPT 逆转了 miR-96-5p 对 LPS 诱导的炎症反应的影响。此外，miR-96-5p 抑制 LPS 刺激的 RAW264.7 细胞中的核因子（NF）-κB 信号通路。miR-96-5p 通过靶向 NAMPT 抑制 NF-κB 通路减轻新生儿败血症中的炎症反应。
