Müller J, Pfeifer U, Dämmrich J
Virchows Arch B Cell Pathol Incl Mol Pathol. 1987;52(5):429-41. doi: 10.1007/BF02889983.
Autophagic vacuoles (AVs) in adrenal parenchymal cells of the outer part of the zona fasciculata were investigated by means of electron microscopic morphometry in 40 adult male Sprague Dawley rats. Four test groups and four control groups received twice daily subcutaneous injections of ACTH (12.5 U/kg body weight) or physiological saline, respectively, for 3, 6, 9 and 12 days. At 12 days the total dry weight of the adrenals was increased in ACTH-treated animals from 17.2 to 71.0 mg (about fourfold) and the average volume of zona fasciculata cells from 1,500 to 5,100 microns3 (about threefold). The relatively larger increase in total mass reflects mitotic activity in the growing adrenal tissue. The calculated growth rate of total tissue expressed as the percentage increase in dry weight per day was 12% at day 3 and decreased to 4.6% at day 12 of ACTH stimulation. During the time interval investigated the cytoplasmic volume fraction of autophagic vacuoles was significantly reduced from 10 X 10(-5) in controls to 1 X 10(-5) in ACTH-treated animals (p less than 0.001). Assuming a half-life for AVs of 7.9 min (Papadopoulos and Pfeifer 1986), autophagic degradation of cytoplasm can be calculated as 1.3%/day in controls, but only 0.15%/day in ACTH-treated animals. Thus growth of 1.15%/day can be attributed to inhibition of autophagic degradation. Although this is only part of total growth, it is evident from the present results that anticatabolic growth induction is utilized nearly to its maximum in adrenocortical fasciculata cells under the influence of ACTH.
通过电子显微镜形态计量学方法，对40只成年雄性Sprague Dawley大鼠束状带外侧肾上腺实质细胞中的自噬泡（AVs）进行了研究。四个试验组和四个对照组分别每日皮下注射两次促肾上腺皮质激素（ACTH，12.5 U/kg体重）或生理盐水，持续3、6、9和12天。在第12天时，接受ACTH治疗的动物肾上腺总干重从17.2毫克增加到71.0毫克（约四倍），束状带细胞的平均体积从1500立方微米增加到5100立方微米（约三倍）。总质量相对较大的增加反映了生长中的肾上腺组织的有丝分裂活性。以干重每日增加百分比表示的总组织计算生长率在ACTH刺激的第3天为12%，在第12天降至4.6%。在所研究的时间间隔内，自噬泡的细胞质体积分数从对照组的10×10⁻⁵显著降低到ACTH治疗动物的1×10⁻⁵（p＜0.001）。假设自噬泡的半衰期为7.9分钟（Papadopoulos和Pfeifer，1986），对照组中细胞质的自噬降解计算为每日1.3%，但在ACTH治疗的动物中仅为每日0.15%。因此，每日1.15%的生长可归因于自噬降解的抑制。虽然这只是总生长的一部分，但从目前的结果可以明显看出，在ACTH的影响下，肾上腺皮质束状带细胞中抗分解代谢生长诱导几乎被充分利用。
