一种新型冷冻探头用于慢性绵羊模型心房消融的评估。
Evaluation of a Novel Cryoprobe for Atrial Ablation in a Chronic Ovine Model.
作者信息
Schill Matthew R, Melby Spencer J, Speltz Molly, Breitbach May, Schuessler Richard B, Damiano Ralph J
机构信息
Division of Cardiothoracic Surgery, Washington University School of Medicine, St. Louis, Missouri.
Scientific Solutions, LLC, Fridley, Minnesota.
出版信息
Ann Thorac Surg. 2017 Sep;104(3):1069-1073. doi: 10.1016/j.athoracsur.2017.02.041.
PURPOSE
Cryoablation is used in the treatment of atrial fibrillation and other cardiac arrhythmias. This study evaluated a novel 10-cm flexible nitrous oxide cryoprobe in an ovine model of atrial ablation.
DESCRIPTION
Six sheep were anesthetized, underwent a left thoracotomy, and were placed on cardiopulmonary bypass. A left atriotomy was performed, and the cryoprobe was applied endocardially for 120 seconds at less than -40°C to 4 sites on the left atrium. The atrium was closed and the animals were allowed to recover. After 30 days, the animals were euthanized. Transmurality was evaluated in 5-mm sections of each lesion using 2,3,5-triphenyltetrazolium chloride (TTC) and Masson's trichrome staining.
EVALUATION
All animals survived. One hundred four of 106 sections (98%) were transmural by TTC; 103 of 106 (97%) sections were transmural by trichrome staining. There was no late atrial perforation, intraluminal thrombus, or thromboembolism.
CONCLUSIONS
The device reliably produced transmural lesions in a chronic ovine model. Its performance was equivalent to that of other nitrous oxide cryoablation systems.
目的
冷冻消融用于治疗心房颤动及其他心律失常。本研究在绵羊心房消融模型中评估了一种新型的10厘米柔性一氧化二氮冷冻探头。
描述
对6只绵羊进行麻醉，实施左胸切开术，并进行体外循环。切开左心房，将冷冻探头在心内膜上于低于-40°C的温度下应用于左心房的4个部位，持续120秒。关闭心房，让动物恢复。30天后，对动物实施安乐死。使用2,3,5-三苯基四氮唑氯化物（TTC）和马松三色染色法在每个病变的5毫米切片中评估透壁性。
评估
所有动物均存活。106个切片中有104个（98%）经TTC染色显示为透壁；106个切片中有103个（97%）经三色染色显示为透壁。未出现晚期心房穿孔、腔内血栓或血栓栓塞。
结论
该装置在慢性绵羊模型中能可靠地产生透壁性病变。其性能与其他一氧化二氮冷冻消融系统相当。
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