Farrer L A, Goodfellow P J, Lamarche C M, Franjkovic I, Myers S, White B N, Holden J J, Kidd J R, Simpson N E, Kidd K K
Am J Hum Genet. 1987 Apr;40(4):329-37.
Members of four families in which multiple endocrine neoplasia type 2A (MEN-2A) is segregating were typed for seven DNA markers and one red cell enzyme marker on chromosome 13. Close linkage was excluded between the MEN2A locus and each marker locus tested. By means of multipoint analysis and the genetic map of chromosome 13 developed by Leppert et al., MEN2A was excluded from any position between the most proximal marker locus (D13S6) and the most distal marker locus (D13S3) and from within 12 cMorgans outside these two loci, respectively. However, the support of exclusion within an interval was diminished under the assumption of a substantially larger genetic map in females. The strategy of multipoint analysis, which excluded between 1.5 and 2.0 times more chromosome 13 than did two-point analysis, demonstrates the utility of linkage maps in mapping disease genes.
对四个有多发性内分泌肿瘤2A型（MEN - 2A）家族成员进行了13号染色体上七个DNA标记和一个红细胞酶标记的分型。MEN2A基因座与每个测试的标记基因座之间排除了紧密连锁。通过多点分析以及Leppert等人绘制的13号染色体遗传图谱，分别将MEN2A排除在最近端标记基因座（D13S6）和最远端标记基因座（D13S3）之间的任何位置，以及这两个基因座之外12厘摩范围内。然而，在假设女性遗传图谱大得多的情况下，区间内排除的支持度有所降低。多点分析策略排除的13号染色体区域比两点分析多1.5至2.0倍，证明了连锁图谱在疾病基因定位中的作用。
