Farrell L B, Nagley P
Biochem Biophys Res Commun. 1987 May 14;144(3):1257-64. doi: 10.1016/0006-291x(87)91446-x.
Clones encoding the proteolipid subunit 9 of the mitochondrial ATPase complex have been isolated from a lambda gt10 library of human liver cDNA sequences, using a probe of Neurospora crassa cDNA encoding subunit 9. From nucleotide sequence analysis it is concluded that the amino acid sequence of mature human subunit 9 is identical to that of its bovine counterpart. By comparing the sequence of two cDNA clones (denoted human 1 and 2) to those of two bovine cDNA clones (denoted P1 and P2) recently described by Gay and Walker (EMBO J. 4, 3519-3524, 1985) it is evident that there are close sequence relationships between human 1 and bovine P1, and between human 2 and bovine P2, although both human clones are truncated at their 5'-ends. Thus, as in bovine cells there appears to be at least two human genes encoding subunit 9.
利用编码线粒体ATP酶复合体蛋白脂质亚基9的粗糙脉孢菌cDNA探针，从人肝脏cDNA序列的λgt10文库中分离出了相关克隆。通过核苷酸序列分析得出，成熟人亚基9的氨基酸序列与其牛对应物的序列相同。将两个cDNA克隆（分别记为人类1和2）的序列与Gay和Walker最近描述的两个牛cDNA克隆（分别记为P1和P2）（《欧洲分子生物学组织杂志》4，3519 - 3524，1985）的序列进行比较，很明显人类1与牛P1之间以及人类2与牛P2之间存在密切的序列关系，尽管两个人类克隆在其5'端都被截短了。因此，与牛细胞一样，人类似乎至少有两个编码亚基9的基因。
