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采用 NaClO 和γ射线辐照联用的方法对去壳生牡蛎(太平洋牡蛎)和贻贝(菲律宾蛤仔)中的副溶血性弧菌进行灭活。

Inactivation of Vibrio parahaemolyticus in shucked raw oyster ( Grassostrea gigas) and clam ( Venerupis phillippinarum) by using a combination of NaClO and gamma irradiation.

机构信息

School of Food Science and Technology, Chung-Ang University, Ansung, Republic of Korea.

出版信息

Food Sci Technol Int. 2018 Jan;24(1):43-52. doi: 10.1177/1082013217726634. Epub 2017 Aug 25.

DOI:10.1177/1082013217726634
PMID:28840748
Abstract

This study investigated the synergistic effects of sodium hypochlorite (NaClO) and gamma irradiation combination against Vibrio parahaemolyticus in shucked oysters and clams. V. parahaemolyticus decreased to 1.1-5.6 log CFU/g in oysters and 1.1-5.7 logCFU/g in clams by NaClO (20-80 ppm) + γ irradiation (0.1-2.0 kGy) combinations. V. parahaemolyticus was not detected by 60 or 80 ppm NaClO + 2.0 kGy. Synergistic reduction of >1 log was observed by 60 ppm NaClO + 0.3-2 kGy and 80 ppm NaClO + 0.5 or 2 kGy. Specifically, >2 log of the synergistic reduction was obtained by 60 or 80 ppm NaClO + 2 kGy. Furthermore, using the Weibull model, 5D values (5-log reductions) were calculated for 60 or 80 ppm NaClO + 0.5-0.9 kGy. No significant differences were observed for all sensory parameters between samples of 2.0 kGy + 0-80 ppm NaClO. This study suggests that 60 ppm NaClO + 2.0 kGy in reducing 7-log V. parahaemolyticus without any deteriorative changes of sensory qualities could be a potential strategy for post-harvest process in seafood processing and distribution to enhance the microbial safety of molluscan shellfish.

摘要

本研究探讨了次氯酸钠(NaClO)和伽马射线联合处理对去壳牡蛎和蛤中副溶血性弧菌的协同作用。在牡蛎中,NaClO(20-80ppm)+γ射线(0.1-2.0kGy)组合可使副溶血性弧菌减少 1.1-5.6 log CFU/g;在蛤中,副溶血性弧菌减少 1.1-5.7 logCFU/g。60 或 80ppm NaClO+2.0kGy 未检出副溶血性弧菌。60ppm NaClO+0.3-2kGy 和 80ppm NaClO+0.5 或 2kGy 观察到协同减少>1 log。60 或 80ppm NaClO+2kGy 可获得>2 log 的协同减少。此外,使用 Weibull 模型,计算了 60 或 80ppm NaClO+0.5-0.9kGy 的 5D 值(5-log 减少)。2.0kGy+0-80ppm NaClO 的所有感官参数之间未观察到显著差异。本研究表明,60ppm NaClO+2.0kGy 可减少 7-log 副溶血性弧菌,而感官质量无任何恶化变化,可能是海产品加工和分配后处理过程中的一种潜在策略,可提高贝类的微生物安全性。

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