细菌凝聚素的生化分析
Biochemical Analysis of Bacterial Condensins.
作者信息
Petrushenko Zoya M, Rybenkov Valentin V
机构信息
Department of Chemistry and Biochemistry, University of Oklahoma, 101 Stephenson Parkway, Norman, OK, 73019, USA.
出版信息
Methods Mol Biol. 2017;1624:145-159. doi: 10.1007/978-1-4939-7098-8_12.
Condensins help establish compactness of bacterial chromosomes and assist in their segregation during cell growth and division. They act as elaborate macromolecular machines that organize the chromosome on a global scale and link it to the pan-cell dynamics. The mechanism of condensins in its entirety is yet to be elucidated. However, many aspects of condensin activity have been recuperated in vitro. This report described purification of the Escherichia coli condensin MukBEF, its reassembly from purified components, and reconstitution of DNA supercoiling and DNA bridging activities of the complex.
凝聚素有助于建立细菌染色体的紧凑性，并在细胞生长和分裂过程中协助其分离。它们作为精巧的大分子机器，在全局范围内组织染色体，并将其与全细胞动态联系起来。凝聚素的整体机制尚待阐明。然而，凝聚素活性的许多方面已在体外得到重现。本报告描述了大肠杆菌凝聚素MukBEF的纯化、从纯化组分进行的重新组装，以及该复合物的DNA超螺旋和DNA桥接活性的重建。
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