有丝分裂检查点基因AURKA中的单核苷酸多态性rs911160和AURKB中的rs2289590与胃癌易感性有关。

Single nucleotide polymorphisms rs911160 in AURKA and rs2289590 in AURKB mitotic checkpoint genes contribute to gastric cancer susceptibility.

作者信息

Mesic Aner, Markocic Ela, Rogar Marija, Juvan Robert, Hudler Petra, Komel Radovan

机构信息

Department of Biology, Faculty of Science, University of Sarajevo, Zmaja od Bosne 33-35, 71000 Sarajevo, Bosnia and Herzegovina.

Institute of Biochemistry, Medical Centre for Molecular Biology, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, SI-1000 Ljubljana, Slovenia.

出版信息

Environ Mol Mutagen. 2017 Dec;58(9):701-711. doi: 10.1002/em.22129. Epub 2017 Aug 26.

DOI:10.1002/em.22129

PMID:28843004

Abstract

BACKGROUND

Single nucleotide polymorphisms (SNPs) in mitotic checkpoint genes could confer increased susceptibility to gastric cancer (GC). We investigated the association of Aurora kinase A (AURKA), Aurora kinase B (AURKB), Aurora kinase C (AURKC), Polo-like kinase 1 (PLK1) and Budding uninhibited by benzimidazol 3, yeast (BUB3) gene polymorphisms with GC risk.

MATERIALS AND METHODS

Genotyping of 6 SNPs in AURKA (rs911160 and rs8173), AURKB (rs2289590), AURKC (rs11084490), PLK1 (rs42873), and BUB3 (rs7897156) was performed using TaqMan genotyping assays.

RESULTS

Our study demonstrated that rs911160 (AURKA) heterozygous genotype was associated with an increased GC risk (OR = 1.50, 95% CI = 1.01-2.22, P = 0.043). Analysis of rs911160 (AURKA) showed significant association with an increased risk for intestinal type GC (OR = 1.80, 95%CI = 1.01-3.21, P = 0.040) and the risk was significantly higher in women than men (OR = 2.65, 95%CI = 1.02-6.87, P = 0.033). SNP rs2289590 in AURKB might contribute to susceptibility for the development of gastric cancer, particularly in women (OR = 2.08, 95% CI = 1.05-4.09, P = 0.032).

CONCLUSION

Our findings suggested that AURKA (rs911160) and AURKB (rs2289590) polymorphisms could affect GC risk. Further validation studies in larger and multi-ethnical populations are needed to elucidate their functional impact on the development of GC. Environ. Mol. Mutagen. 58:701-711, 2017. © 2017 Wiley Periodicals, Inc.

摘要

背景

有丝分裂检查点基因中的单核苷酸多态性（SNP）可能会增加患胃癌（GC）的易感性。我们研究了极光激酶A（AURKA）、极光激酶B（AURKB）、极光激酶C（AURKC）、波罗蛋白样激酶1（PLK1）和酵母中苯并咪唑3未抑制的出芽蛋白（BUB3）基因多态性与GC风险的关联。

材料与方法

使用TaqMan基因分型检测法对AURKA（rs911160和rs8173）、AURKB（rs2289590）、AURKC（rs11084490）、PLK1（rs42873）和BUB3（rs7897156）中的6个SNP进行基因分型。

结果

我们的研究表明，rs911160（AURKA）杂合基因型与GC风险增加相关（OR = 1.50，95% CI = 1.01 - 2.22，P = 0.043）。对rs911160（AURKA）的分析显示，其与肠型GC风险增加显著相关（OR = 1.80，95%CI = 1.01 - 3.21，P = 0.040），且女性的风险显著高于男性（OR = 2.65，95%CI = 1.02 - 6.87，P = 0.033）。AURKB中的SNP rs2289590可能会增加患胃癌的易感性，尤其是在女性中（OR = 2.08，95% CI = 1.05 - 4.09，P = 0.032）。