Department of Orthopedics, Huizhou Medical Research Center, Huizhou 1st People's Hospital, Huizhou, Guangdong, China (mainland).
Department of Orthopedics, Longmen People's Hospital, Huizhou, Guangdong, China (mainland).
Med Sci Monit. 2017 Aug 27;23:4132-4140. doi: 10.12659/msm.903427.
BACKGROUND Leptocarpin (LTC) has drawn much attention for suppressing tumor growth or reducing inflammation. However, the effect of LTC on osteosarcoma has rarely been reported. Our object was to determine whether LTC suppresses MG63 cell proliferation, migration, and invasion, and whether type-1 insulin-like growth factor receptor (IGF-1R) is one of the targets in LTC suppressing osteosarcoma. MATERIAL AND METHODS Cytotoxicity of LTC was performed by use of a cell-counting kit-8 (CCK-8). RNA interference (RNAi) or pEABE-bleo IGF-1R plasmid were used for silencing or overexpressing IGF-1R, Western blot (WB) analysis was used for IGF-1R expression, CCK-8 for proliferation, and transwell assay for migration and invasion. RESULTS LTC (23.533 μM) treatment for 48 h was taken as the 50% inhibiting concentration (IC50), which significantly (P<0.05) suppressed MG63 cells proliferation, migration, and invasion. LTC (IC50) obviously inhibited IGF-1R expression in MG63 cells, with similar effect to small interfering RNA (siRNA), while pEABE-bleo IGF-1R transfection overexpressed IGF-1R. siRNA silencing IGF-1R suppressed MG63 cells proliferation, migration, and invasion, while pEABE-bleo IGF-1R transfection was significantly (P<0.05) promoted. With or without siRNA or pEABE-bleo IGF-1R transfection, LTC (IC50) suppressed MG63 cells proliferation, migration, and invasion. The effect of LTC (IC50) combined with siRNA on suppressing MG63 cells proliferation, migration, and invasion was more obvious, while the effect of LTC (IC50) combined with pEABE-bleo IGF-1R transfection was less significant (P<0.05). CONCLUSIONS LTC suppressed osteosarcoma proliferation, migration, and invasion by inhibiting IGF-1R expression. IGF-1R is one of the targets in LTC suppressing osteosarcoma.
莱菔硫烷（LTC）在抑制肿瘤生长或减轻炎症方面引起了广泛关注。然而，LTC 对骨肉瘤的作用鲜有报道。我们的目的是确定 LTC 是否抑制 MG63 细胞的增殖、迁移和侵袭，以及是否胰岛素样生长因子 1 型受体（IGF-1R）是 LTC 抑制骨肉瘤的靶标之一。
使用细胞计数试剂盒-8（CCK-8）测定 LTC 的细胞毒性。采用 RNA 干扰（RNAi）或 pEABE-bleo IGF-1R 质粒沉默或过表达 IGF-1R，Western blot（WB）分析用于 IGF-1R 表达，CCK-8 用于增殖，transwell 测定用于迁移和侵袭。
48 h 时 LTC（23.533 μM）处理被视为 50%抑制浓度（IC50），显著（P<0.05）抑制 MG63 细胞的增殖、迁移和侵袭。LTC（IC50）明显抑制 MG63 细胞中的 IGF-1R 表达，作用与小干扰 RNA（siRNA）相似，而 pEABE-bleo IGF-1R 转染则过表达 IGF-1R。siRNA 沉默 IGF-1R 抑制 MG63 细胞的增殖、迁移和侵袭，而 pEABE-bleo IGF-1R 转染则显著（P<0.05）促进。无论是否进行 siRNA 或 pEABE-bleo IGF-1R 转染，LTC（IC50）均抑制 MG63 细胞的增殖、迁移和侵袭。LTC（IC50）联合 siRNA 抑制 MG63 细胞增殖、迁移和侵袭的效果更为明显，而 LTC（IC50）联合 pEABE-bleo IGF-1R 转染的效果则不明显（P<0.05）。
LTC 通过抑制 IGF-1R 表达抑制骨肉瘤的增殖、迁移和侵袭。IGF-1R 是 LTC 抑制骨肉瘤的靶标之一。
