a Department of Medicine, School of Medicine , Universidad Complutense de Madrid , Madrid , Spain.
b Surgery Department , Hospital Universitario ROF-Codina , Lugo , Spain.
Arch Physiol Biochem. 2018 Feb;124(1):54-60. doi: 10.1080/13813455.2017.1360913. Epub 2017 Aug 26.
Heat generation by brown adipose tissue (BAT) in response to temperature reduction seems to be entirely related to sympathetic nervous stimulation.
To analyse if temperature reduction and norepinephrine may differently affect the expression of proteins related to energy metabolism in BAT.
Isolated rats BAT was incubated with/without norepinephrine (10 mol/L, 24 h at 32 °C and 37 °C).
In BAT, 32 °C increased the protein expression levels of carnitine palmitoyltransferase-I and -II, mitochondrial uncoupling protein-1 (UCP-1) and the expression and activity of lactate dehydrogenase. Mitochondrial F-ATP synthase α-chain expression was decreased at 32 °C compared to 37 °C. Norepinephrine and at 32 °C exposure, UCP-1 expression was increased but cytochrome-c oxidase and F-ATP synthase α-chain expression was reduced with respect to 37 °C.
Sympathetic stimulation seems not to be the only factor associated with heat generation.
Temperature reduction by itself exerts some different effects on the expression of proteins related to the energy metabolism than norepinephrine.
棕色脂肪组织（BAT）在响应温度降低时产生热量似乎完全与交感神经刺激有关。
分析温度降低和去甲肾上腺素是否可能以不同的方式影响 BAT 中与能量代谢相关的蛋白质的表达。
分离大鼠的 BAT，在 32°C 和 37°C 下用/不用去甲肾上腺素（10 mol/L，24 小时孵育）孵育。
在 BAT 中，32°C 增加了肉毒碱棕榈酰转移酶-I 和 -II、线粒体解偶联蛋白-1（UCP-1）以及乳酸脱氢酶的表达和活性。与 37°C 相比，32°C 时线粒体 F-ATP 合酶α链的表达降低。与 37°C 相比，去甲肾上腺素和 32°C 暴露时 UCP-1 的表达增加，但细胞色素-c 氧化酶和 F-ATP 合酶α链的表达减少。
交感神经刺激似乎不是与产热相关的唯一因素。
与去甲肾上腺素相比，温度降低本身对与能量代谢相关的蛋白质的表达有一些不同的影响。
