Riou Cindy, Cordeiro Luiz, Gérard Nadine
INRA, UMR INRA 85, UMR CNRS 7247, Université de Tours, IFCE, Physiologie de la Reproduction et des Comportements, Interactions Cellulaires et Fertilité, 37380 Nouzilly, France; ALLICE, Station de Phénotypage, Lieu-Dit Le Perroi, 37380 Nouzilly, France.
INRA, UMR INRA 85, UMR CNRS 7247, Université de Tours, IFCE, Physiologie de la Reproduction et des Comportements, Interactions Cellulaires et Fertilité, 37380 Nouzilly, France; Federal University of Semi Arid Region, Mossoro, 59625-900, Rio Grande do Norte, Brazil.
Anim Reprod Sci. 2017 Oct;185:28-41. doi: 10.1016/j.anireprosci.2017.07.022. Epub 2017 Aug 12.
While uterine epithelium secretes eggshell matrix proteins to regulate eggshell structural organization, uterovaginal junction (UVJ) epithelium supports sperm storage in tubules (SST). Here, we examined the presence of OCX36, OC-116 and OC-17 eggshell matrix proteins in SSTs. Two experimental lines of hens displaying either a long (F+ line) or a short (F- line) potential to store sperm were used, before and 24h after insemination. Using immunohistochemistry and western blot, we analyzed the presence of OC-116, OC-17 and OCX36 proteins in the SSTs. Using lectin and calcium staining, we examined the presence in SSTs of Gal/GalNAc (Galactose/N-acetylgalactosamine) and Glc/GlcNAc (Glucose/N-acetylglucosamine) glycans, as well as calcium ions. Our results indicate that in both F+ and F- hens, the eggshell matrix proteins OC-116 and OCX36 were identified in SST cells and lumen, in contact with spermatozoa. The OC-17 protein was found associated with calcium in F+ and F- hens, only in the SST lumen 24h after insemination. Glycans Gal/GalNAc and Glc/GlcNAc were found to be more abundant in the apical cytoplasmic area of the SST cells of F+ hens than in that of F- hens after insemination. This is the first report demonstrating the presence in SSTs of the OC-116, OC-17 and OCX36 eggshell matrix proteins, and their concomitant presence with Gal/GalNAc and Glc/GlcNAc glycans, as well as with calcium. Our results suggest that the OC-116, OC-17 and OCX36 eggshell matrix proteins may be involved in sperm storage.
子宫上皮分泌蛋壳基质蛋白以调节蛋壳的结构组织，而子宫阴道连接部（UVJ）上皮则支持精子在小管中储存（SST）。在此，我们检测了SST中OCX36、OC - 116和OC - 17蛋壳基质蛋白的存在情况。使用了两个实验品系的母鸡，它们在储存精子方面分别表现出长（F +系）或短（F -系）的潜力，在授精前和授精后24小时进行研究。通过免疫组织化学和蛋白质印迹法，我们分析了SST中OC - 116、OC - 17和OCX36蛋白的存在情况。使用凝集素和钙染色，我们检测了SST中半乳糖/ N - 乙酰半乳糖胺（Gal/GalNAc）和葡萄糖/ N - 乙酰葡糖胺（Glc/GlcNAc）聚糖以及钙离子的存在情况。我们的结果表明，在F +和F -母鸡中，蛋壳基质蛋白OC - 116和OCX36在SST细胞和管腔中被鉴定出来，与精子接触。在F +和F -母鸡中，仅在授精后24小时，OC - 17蛋白在SST管腔中被发现与钙相关。授精后，发现F +母鸡SST细胞顶端细胞质区域中的Gal/GalNAc和Glc/GlcNAc聚糖比F -母鸡中的更丰富。这是首次报道证明SST中存在OC - 116、OC - 17和OCX36蛋壳基质蛋白，以及它们与Gal/GalNAc和Glc/GlcNAc聚糖以及钙同时存在。我们的结果表明，OC - 116、OC - 17和OCX36蛋壳基质蛋白可能参与精子储存。
