Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian, Liaoning, 116023, PR China.
Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian, Liaoning, 116023, PR China.
Fish Shellfish Immunol. 2017 Oct;69:218-226. doi: 10.1016/j.fsi.2017.08.027. Epub 2017 Aug 26.
The ras-related C3 botulinum toxin substrate 1 (Rac1) belongs to Ras homolog (Rho) small GTPases subfamily. As an important molecular switch, Rac1 regulates various processes in the cell, especially in cellular immune response. With attempt to clarify characters and functions of Rac1 in sea cucumbers, full length cDNA of a Rac1 homolog in the sea cucumber Apostichopus japonicus (AjRac1) was cloned by transcriptome database mining and rapid amplification of cDNA ends (RACE) techniques. The open reading frame of AjRac1 is 579 bp encoding a protein with a length of 192 aa. Sequence analysis showed that AjRac1 is highly conserved as compared to those from other eukaryotic species. Phylogenetic analysis revealed that amino acid sequence of AjRac1 closely related to those from Strongylocentrotus purpuratus. Results of expression analysis showed that AjRac1 exhibited a relative high expression in blastula stage, adult coelomocytes and respiratory tree in A. japonicus. The transcription of AjRac1 in adult coelomocytes altered significantly at 4 h- and 12 h-after Vibrio splendidus infection, respectively, which indicated that AjRac1 involved in sea cucumber innate immunity. All data presented in this study will deepen our understanding of characterizations and immunological functions of Rac1 in sea cucumbers.
Ras 相关 C3 肉毒杆菌毒素底物 1（Rac1）属于 Ras 同源物（Rho）小 GTP 酶亚家族。作为一个重要的分子开关，Rac1 调节细胞中的各种过程，特别是在细胞免疫反应中。为了阐明海参 Rac1 的特征和功能，我们通过转录组数据库挖掘和快速扩增 cDNA 末端（RACE）技术克隆了海参刺参（AjRac1）的 Rac1 同源物全长 cDNA。AjRac1 的开放阅读框为 579bp，编码一个长度为 192aa 的蛋白质。序列分析表明，AjRac1 与其他真核生物相比高度保守。系统进化分析显示，AjRac1 的氨基酸序列与强壮轮虫（Strongylocentrotus purpuratus）的氨基酸序列密切相关。表达分析结果表明，AjRac1 在囊胚期、成体体腔细胞和呼吸树中表达水平相对较高。AjRac1 在成体体腔细胞中的转录在 Vibrio splendidus 感染后 4h 和 12h 时分别发生显著变化，表明 AjRac1 参与了海参先天免疫。本研究中提供的所有数据将加深我们对海参 Rac1 特征和免疫学功能的理解。
