Sebbag Lionel, Harrington Danielle M, Mochel Jonathan P
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
Lloyd Veterinary Medical Center, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
Vet Ophthalmol. 2018 May;21(3):249-254. doi: 10.1111/vop.12502. Epub 2017 Aug 27.
To compare the use of two ophthalmic sponges for tear collection in dogs and cats.
Ten healthy dogs and 10 healthy cats.
A strip (4 × 10 mm) of either cellulose or polyvinyl acetal (PVA) sponge was inserted into the ventral fornix of each eye for either 15, 30, or 60 s. The wetted strip was placed into a 0.2-mL tube that was first punctured at its bottom. Tears were eluted through the drainage hole into a 1.5-mL tube via centrifugation. Tear volume absorbed (VA) and tear volume recovered (VR) were calculated as the difference of the post- and precollection weight of the 0.2-mL tube and 1.5-mL tube, respectively. Recovery ratio (RR) was determined as the ratio between VR and VA.
Ophthalmic sponges were well tolerated by all subjects. In dogs and cats, median (95% range) VA, VR, and RR were as follows: 44 μL (11-106 μL) and 16 μL (2-43 μL); 27 μL (1-84 μL) and 6 μL (0-29 μL); 64% (7-91%) and 35% (0-86%), respectively. PVA sponges achieved significantly greater VR in cats and RR in both species. All parameters were significantly greater with a collection time of 60 vs. 30 and 15 s. Body weight was associated with VA and VR in dogs but not cats.
Polyvinyl acetal is better than cellulose for tear collection given its superior recovery. Ophthalmic sponges could facilitate routine analysis of tear fluid in dogs and cats, although further studies are needed to evaluate the quality of tears obtained with this method.
比较两种眼科海绵在犬猫泪液采集中的应用。
10只健康犬和10只健康猫。
将一条（4×10毫米）纤维素或聚乙烯醇缩醛（PVA）海绵插入每只眼睛的下穹窿，持续15、30或60秒。将浸湿的海绵条放入一个预先在底部打孔的0.2毫升试管中。通过离心，泪液经排水孔洗脱到一个1.5毫升试管中。分别计算0.2毫升试管和1.5毫升试管采集前后重量差，得出吸收泪液体积（VA）和回收泪液体积（VR）。回收率（RR）定义为VR与VA的比值。
所有受试动物对眼科海绵耐受性良好。犬猫的VA、VR和RR中位数（95%范围）如下：44微升（11 - 106微升）和16微升（2 - 43微升）；27微升（1 - 84微升）和6微升（0 - 29微升）；64%（7 - 91%）和35%（0 - 86%）。PVA海绵在猫中实现了显著更高的VR，在两个物种中RR均更高。采集时间为60秒时，所有参数均显著高于30秒和15秒。犬的体重与VA和VR相关，而猫则不然。
鉴于聚乙烯醇缩醛回收率更高，其在泪液采集方面优于纤维素。眼科海绵有助于犬猫泪液的常规分析，不过仍需进一步研究评估该方法获取的泪液质量。
