Wang L, Liu X Z, Ren Z Y, Ding L, Nan J, Liu C L, Song Z C, Feng M J, Yang Q, Wang J T
Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan 030001, China.
Community Health Center, Shanxi Cardiovascular Hospital, Taiyuan 030001, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2017 Aug 10;38(8):1113-1117. doi: 10.3760/cma.j.issn.0254-6450.2017.08.023.
To explore the effect of polycyclic aromatic hydrocarbons (PAHs) and gene CpG island methylation, as well as their interaction in cervical intraepithelial neoplasias. Objects of this study were from a cohort of cervical lesions study in Yangqu county of Shanxi province. All the patients were diagnosed pathologically, that including 83 patients with high-grade cervical intraepithelial neoplasia (CINⅡ/Ⅲ), 86 patients with low-grade cervical intraepithelial neoplasia (CINⅠ) and another 91 women under normal cervical (NC) condition. 1-hydroxy pyrene in the urine was detected by high performance liquid chromatography (HPLC) while CpG island methylation status of tumor suppressor gene and were measured by methylation-specifc polymerase chain reaction (MSP). Data were analyzed with Kruskal-Wallis test, chi-square test and trend of chi-square test. Logistic regression models were used to estimate the odds ratio () and corresponding 95% confidence intervals (95%) between influencing factors and the cervical disease by using the SPSS statistical software (version 20.0). The interaction under study was evaluated by using the generalized multifactor dimensionality reduction (GMDR) model. Level of 1-hydroxy pyrene (=50.743, <0.001) and the high exposure rate of 1-hydroxy pyrene (trend (2)=20.146, <0.001) were gradually increasing along with the severity of cervical intraepithelial neoplasia. The CpG island methylation rates of in CINⅠand CINⅡ/Ⅲ group were higher than that in NC group, and gradually increasing along with the severity of cervical intraepithelial neoplasia (trend (2)=9.75, =0.002; trend (2)=10.39, =0.001). Results from the GMDR model showed that interaction existed among the high exposure of 1-hydroxy pyrene and the CpG island methylation of in CINⅠ and CINⅡ/Ⅲ group. Under the high exposure of 1-hydroxy pyrene and the CpG island methylation of appeared to have increased the risk of cervical intraepithelial neoplasia and causing synergistic effect in cervical intraepithelial neoplasia.
探讨多环芳烃（PAHs）与基因CpG岛甲基化及其相互作用在宫颈上皮内瘤变中的影响。本研究对象来自山西省阳曲县的一组宫颈病变研究队列。所有患者均经病理诊断，其中包括83例高级别宫颈上皮内瘤变（CINⅡ/Ⅲ）患者、86例低级别宫颈上皮内瘤变（CINⅠ）患者以及另外91例宫颈正常（NC）的女性。采用高效液相色谱法（HPLC）检测尿液中的1-羟基芘，同时采用甲基化特异性聚合酶链反应（MSP）检测抑癌基因的CpG岛甲基化状态。数据采用Kruskal-Wallis检验、卡方检验和卡方趋势检验进行分析。使用SPSS统计软件（版本20.0），通过逻辑回归模型估计影响因素与宫颈疾病之间的比值比（OR）及相应的95%置信区间（95%CI）。采用广义多因素降维（GMDR）模型评估所研究的相互作用。1-羟基芘水平（χ² = 50.743，P < 0.001）和1-羟基芘高暴露率（趋势χ²(2) = 20.146，P < 0.001）随着宫颈上皮内瘤变的严重程度逐渐升高。CINⅠ组和CINⅡ/Ⅲ组中基因的CpG岛甲基化率高于NC组，且随着宫颈上皮内瘤变的严重程度逐渐升高（趋势χ²(2) = 9.75，P = 0.002；趋势χ²(2) = 10.39，P = 0.001）。GMDR模型结果显示，CINⅠ组和CINⅡ/Ⅲ组中1-羟基芘高暴露与基因的CpG岛甲基化之间存在相互作用。在1-羟基芘高暴露和基因的CpG岛甲基化情况下，似乎增加了宫颈上皮内瘤变的风险，并在宫颈上皮内瘤变中产生协同效应。
