College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China; Bioenergy Research Institute, Nanjing Tech University, Nanjing, Jiangsu 211816, China.
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China.
Bioresour Technol. 2017 Nov;244(Pt 1):865-871. doi: 10.1016/j.biortech.2017.07.146. Epub 2017 Jul 27.
The aim of this work was to investigate the mesophilic methane fermentation of rice straw at different organic loading rates (OLRs) in a 300m bioreactor. It was found that biogas production increased when the OLR was below 2.00kg VS/(m·d). The average volumetric biogas production reached 0.86m/(m·d) at an OLR of 2.00kg VS/(m·d). Biogas production rate was 323m/t dry rice straw over the whole process. The pH, chemical oxygen demand, volatile fatty acid, and NH-N concentrations were all in optimal range at different OLRs. High-throughput sequencing analysis indicated that Firmicutes, Fibrobacteres, and Spirochaetes predominated in straw samples. Chloroflexi, Proteobacteria, and Planctomycetes were more abundant in the slurry. The hydrogenotrophic pathway was the main biochemical pathway of methanogenesis in the reactor. This study provides new information regarding the OLR and the differences in the spatial distribution of specific microbiota in a rice straw biogas plant.
本工作旨在研究不同有机负荷率（OLR）下 300m 生物反应器中水稻秸秆的中温甲烷发酵。结果发现，OLR 低于 2.00kg VS/（m·d）时，沼气产量增加。在 OLR 为 2.00kg VS/（m·d）时，平均体积沼气产量达到 0.86m/（m·d）。整个过程中，每干稻草吨产沼气 323m³。在不同 OLR 下，pH 值、化学需氧量、挥发性脂肪酸和 NH-N 浓度均处于最佳范围。高通量测序分析表明，在秸秆样品中，厚壁菌门、纤维杆菌门和螺旋体门占优势。在泥浆中，绿弯菌门、变形菌门和浮霉菌门更为丰富。产氢途径是反应器中甲烷生成的主要生化途径。本研究为水稻秸秆沼气工程的 OLR 以及特定微生物区系在空间分布上的差异提供了新的信息。
