Jumaah Firas, Jędrkiewicz Renata, Gromadzka Justyna, Namieśnik Jacek, Essén Sofia, Turner Charlotta, Sandahl Margareta
Department of Chemistry, Centre for Analysis and Synthesis (CAS), Lund University , P.O. Box 124, 22100 Lund, Sweden.
Department of Analytical Chemistry, Faculty of Chemistry, Gdańsk University of Technology , 11/12 Narutowicza Street, 80-233 Gdańsk, Poland.
J Agric Food Chem. 2017 Sep 20;65(37):8220-8228. doi: 10.1021/acs.jafc.7b02857. Epub 2017 Sep 11.
This study demonstrates the effect of column selectivity and density of supercritical carbon dioxide (SC-CO) on the separation of monochloropropanediol (MCPD) esters, known as food toxicants, using SC-CO without addition of cosolvent in ultrahigh performance supercritical fluid chromatography-mass spectrometry (UHPSFC-MS). This study shows that over 20 2-monochloropropanediol (2-MCPD) and 3-monochloropropanediol (3-MCPD) mono- and diesters are separated on a 2-picolylamine column in less than 12 min. The presence and position of a hydroxyl group in the structure, the number of unsaturated bonds, and the acyl chain length play a significant role in the separation of MCPD esters. The flow rate, backpressure, and column oven temperature, which affect the density of the mobile phase, were shown to have a substantial impact on retention, efficiency, and selectivity. The developed method was successfully applied for the determination of MCPD esters in refined oils and showed a close to excellent green analysis score using the Analytical Eco-Scale.
