Ghosh Debraj, De Rajat K
Machine Intelligence Unit, Indian Statistical Institute, Kolkata. India
Endocr Metab Immune Disord Drug Targets. 2017;17(3):182-188. doi: 10.2174/1871530317666170828111113.
Glycolytic activity during Crabtree effect is similar to that in tumor cells. Research regarding Crabtree effect is very much crucial.
The mechanism of metabolic activities in glycolysis pathway and oxidative phosphorylation pathway in regards to Crabtree effect in Saccharomyces cerevisiae was studied in this paper. We also explored the effects of hexose phosphates in the activities of respiratory chain complexes (III and IV) in inhibition of respiration. Besides, the enhancement of fermentation in response to excess glucose concentration was studied. We discussed the significance of Crabtree effect in mammalian cancer in terms of Crabtree effect in a Crabtree positive organism, as it is similar to cancer metabolism in mammalian cells. We developed an in silico model of Crabtree effect.
A comparative study was performed with laboratory experiments regarding inhibitory role of fructose 1,6-bisphosphate on metabolic respiration. The model was simulated for different concentration levels of glucose and hexose phosphates using COPASI and SNOOPY tools.
We have shown that a hike in glucose concentration increases ethanol concentration and leads glycolytic activity towards fermentation. This phenomenon occurs during Crabtree effect.
克奈特效应期间的糖酵解活性与肿瘤细胞中的相似。关于克奈特效应的研究非常关键。
本文研究了酿酒酵母中与克奈特效应相关的糖酵解途径和氧化磷酸化途径中的代谢活动机制。我们还探讨了磷酸己糖对呼吸链复合物（III和IV）活性在抑制呼吸方面的影响。此外，研究了对过量葡萄糖浓度的发酵增强作用。鉴于克奈特效应在克奈特阳性生物体中与哺乳动物细胞中的癌症代谢相似，我们讨论了克奈特效应在哺乳动物癌症中的意义。我们开发了一个克奈特效应的计算机模型。
针对1,6 - 二磷酸果糖对代谢呼吸的抑制作用进行了实验室实验的比较研究。使用COPASI和SNOOPY工具对不同葡萄糖和磷酸己糖浓度水平的模型进行了模拟。
我们已经表明，葡萄糖浓度的升高会增加乙醇浓度，并使糖酵解活性转向发酵。这种现象在克奈特效应期间发生。
