Habibah Aulidya N, Pfennig Frank, Wilting Joerg, Holtz Wolfgang, Hoerstgen-Schwark Gabriele, Wessels Stephan
Division of Aquaculture and Water Ecology, Department of Animal Sciences, Georg-August-University Goettingen, Goettingen, Germany.
Sex Dev. 2017;11(4):217-224. doi: 10.1159/000479396. Epub 2017 Aug 22.
In teleosts, elevated temperature during embryogenesis can act on germline cell development, which in turn plays a role for sexual fate. In Nile tilapia, a species with high-temperature-induced masculinization, little is known about the effects of increased temperature on gonadal development in non-masculinized females. The aim of the present work was to investigate persistent effects on the germline of genetically female (XX) Nile tilapia reared at normal (28°C) or elevated temperature (36°C) during the critical time of gonadal sex differentiation at 10 to 20 days post fertilization. Non-sex-reversed females were compared to control females to determine persistent effects of temperature on subsequent ovarian development using histological approaches. Germline stem cells were identified using the germline marker Vasa in combination with the proliferation marker PCNA. Vasa- and PCNA-positive germline stem cells were found in ovaries of both high-temperature-treated and control females. In both groups, ovarian germline stem cells were located at the germinal epithelium of the ovigerous lamellae. Although no detrimental effects of high temperature on gonadal development in female Nile tilapia were observed, implications on the reproductive fitness caused by elevated temperature need to be investigated in greater depth.
在硬骨鱼类中，胚胎发育期间的高温可作用于生殖细胞发育，进而对性别命运产生影响。在尼罗罗非鱼这种存在高温诱导雄性化现象的物种中，对于温度升高对未发生雄性化的雌性性腺发育的影响知之甚少。本研究的目的是调查在受精后10至20天性腺性别分化的关键时期，在正常温度（28°C）或高温（36°C）下饲养的遗传雌性（XX）尼罗罗非鱼的生殖细胞所受到的持续性影响。将未发生性逆转的雌性与对照雌性进行比较，采用组织学方法确定温度对后续卵巢发育的持续性影响。使用生殖细胞标记物Vasa结合增殖标记物PCNA来鉴定生殖干细胞。在高温处理的雌性和对照雌性的卵巢中均发现了Vasa和PCNA阳性的生殖干细胞。在两组中，卵巢生殖干细胞均位于产卵板的生发上皮。尽管未观察到高温对尼罗罗非鱼雌性性腺发育有有害影响，但高温对生殖适应性的影响仍需更深入地研究。
