Monobe Marina Mitie, da Silva Rodrigo Costa, Araujo Junior João Pessoa, Takahira Regina Kiomi
Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, 240 Wise Center Drive, Mississippi State, MS 39762-6100 USA.
Department of Microbiology and Immunology, Biosciences Institute, Sao Paulo State University, Botucatu, Brazil.
J Parasit Dis. 2017 Sep;41(3):805-808. doi: 10.1007/s12639-017-0892-8. Epub 2017 Feb 18.
A 6-year-old Labrador with history of hyperpnea, respiratory distress, ascites and paraparesis, presented microfilariae in the blood smear, cytology of the abdominal fluid and urine. The positive samples were confirmed by multiplex-PCR (COI gene) and sequencing, and presented 98% homology to (GenBank accession nos. KC107805.1, EU159111.1, EU163945.1, DQ358815.1, and AJ537512-1). Based on the presented results and the physiopathology of the heartworm disease, molecular techniques may be helpful and accurate in the diagnosis of the disease in vertebrates, and microfilaruria, even uncommon, should be considered on a renal failure evaluation.
一只6岁的拉布拉多犬,有呼吸急促、呼吸窘迫、腹水和轻截瘫病史,血液涂片、腹腔积液和尿液细胞学检查发现微丝蚴。阳性样本通过多重聚合酶链反应(COI基因)和测序得到确认,与(GenBank登录号KC107805.1、EU159111.1、EU163945.1、DQ358815.1和AJ537512-1)具有98%的同源性。基于所呈现的结果以及犬心丝虫病的生理病理学,分子技术在脊椎动物疾病诊断中可能是有用且准确的,即使微丝蚴尿不常见,在评估肾衰竭时也应予以考虑。