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动物过敏性结膜炎抗组胺药物研究模型

Model for Studying Anti- Allergic Drugs for Allergic Conjunctivitis in Animals.

作者信息

Nakazawa Yosuke, Oka Mikako, Takehana Makoto

机构信息

Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato-ku, Tokyo105-8512, 105-8512, Japan.

Laboratory of Clinical Pharmacology, Yokohama University of Pharmacy, Yokohama, Kanagawa, 245-0066, Japan.

出版信息

Open Med (Wars). 2017 Aug 1;12:231-238. doi: 10.1515/med-2017-0034. eCollection 2017.

DOI:10.1515/med-2017-0034
PMID:28848937
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5545756/
Abstract

UNLABELLED

Allergic conjunctivitis (AC), which is characterized by ocular itching, hyperemia, and edema, deteriorates quality of life. In this study, effects of anti-allergic drugs were evaluated by assessing eye-scratching behavior, the number of eosinophils in conjunctiva epithelial tissues, and concentrations of chemical mediators in the tears of the guinea pig model of ovalbumin (OA)-induced AC.

METHODOLOGY

On day 0, 3-week-old guinea pigs were sensitized by OA subconjunctival injections. On days 15, 17, and 19, OA solution was administered. Anti-allergic eye drops were administered 5 and 15 min before the final OA challenge on day 19. Scratching behavior within 1 h after OA exposure was studied. Eosinophils in the conjunctiva were stained with Giemsa reagent. Histamine and substance P (SP) concentrations in tears were measured using ELISA.

RESULTS

Subconjunctivally injected guinea pigs were observed for clinical symptoms. Scratching responses significantly reduced with ketotifen or olopatadine treatment. Eosinophil numbers reduced in animals treated with ketotifen, levocabastine, or tranilast. Histamine and/or SP concentrations in tears were inhibited by some of these anti-allergic drugs.

CONCLUSIONS

It is important to assess the anti-allergic AC drugs objectively because there are several of these drugs currently available. This model allows for an objective evaluation of anti-allergic drugs for AC.

摘要

未标记

过敏性结膜炎（AC）以眼部瘙痒、充血和水肿为特征，会降低生活质量。在本研究中，通过评估卵清蛋白（OA）诱导的AC豚鼠模型的眼部搔抓行为、结膜上皮组织中的嗜酸性粒细胞数量以及泪液中化学介质的浓度，来评价抗过敏药物的效果。

方法

在第0天，对3周龄的豚鼠进行OA结膜下注射致敏。在第15、17和19天，给予OA溶液。在第19天最后一次OA激发前5分钟和15分钟给予抗过敏滴眼液。研究OA暴露后1小时内的搔抓行为。结膜中的嗜酸性粒细胞用吉姆萨试剂染色。使用酶联免疫吸附测定法测量泪液中的组胺和P物质（SP）浓度。

结果

观察结膜下注射豚鼠的临床症状。用酮替芬或奥洛他定治疗后搔抓反应显著降低。用酮替芬、左卡巴斯汀或曲尼司特治疗的动物嗜酸性粒细胞数量减少。这些抗过敏药物中的一些抑制了泪液中的组胺和/或SP浓度。

结论

由于目前有多种抗过敏AC药物，客观评估这些药物很重要。该模型允许对AC的抗过敏药物进行客观评估。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb82/5545756/6773c28af1f9/med-12-231-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb82/5545756/25b1c5e3cc7d/med-12-231-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb82/5545756/233ceabd228c/med-12-231-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb82/5545756/5ab30ac8f827/med-12-231-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb82/5545756/07a17203e5c3/med-12-231-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb82/5545756/6773c28af1f9/med-12-231-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb82/5545756/25b1c5e3cc7d/med-12-231-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb82/5545756/233ceabd228c/med-12-231-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb82/5545756/5ab30ac8f827/med-12-231-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb82/5545756/07a17203e5c3/med-12-231-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb82/5545756/6773c28af1f9/med-12-231-g005.jpg

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