Department of Microbiology, School of Medicine, Ewha Womans University, Seoul 07985, Republic of Korea.
School of Biomedical Sciences, University of Queensland, Brisbane, QLD 4072, Australia.
Mol Med Rep. 2017 Nov;16(5):6170-6177. doi: 10.3892/mmr.2017.7335. Epub 2017 Aug 23.
Mesenchymal stem cells (MSCs) are often considered to be a good source for the development of regenerative medicine. Previously, we reported that tonsil‑derived MSC conditioned medium (T‑MSC CM) produces visceral fat reducing effects. As reduced visceral adiposity is closely associated with an increase in circulating adiponectin, the present study investigated the effects of T‑MSC CM on adiponectin production. T‑MSC CM was collected from previously isolated and characterized T‑MSCs and injected into senescence‑accelerated mouse prone 6 mice, which exhibit characteristics of aging and obesity. The results demonstrated a reduction in mouse weight and epididymal adipose tissue (eAT) mass following injection of T‑MSC CM. Significant increases in adiponectin expression in the eAT, and total and high molecular weight (HMW) adiponectin in the circulation were observed in the T‑MSC CM‑injected mice compared with control mice using reverse transcription‑quantitative polymerase chain reaction, western blot analysis and ELISA. In 3T3‑L1 adipocytes, T‑MSC CM treatment increased adiponectin secretion and multimerization, as detected using western blotting under non‑reducing and non‑heat‑denaturing conditions. Furthermore, glucose oxidase was used to induce oxidative stress in 3T3‑L1 adipocytes and it was observed that T‑MSC CM reduced reactive oxygen species production and the expression of certain oxidative stress markers. In addition, the results also demonstrated that the production of HMW adiponectin was increased, which indicates that T‑MSC CM may enhance adiponectin multimerization via amelioration of oxidative stress. Further studies are required to elucidate anti‑oxidant molecules secreted from T‑MSCs, and these results highlight the potential therapeutic relevance of T‑MSC CM for the treatment of obesity or obesity‑associated diseases.
间充质干细胞（MSCs）通常被认为是再生医学发展的良好来源。先前，我们报道了扁桃体来源的 MSC 条件培养基（T-MSC CM）可产生减少内脏脂肪的作用。由于内脏脂肪减少与循环脂联素的增加密切相关，因此本研究探讨了 T-MSC CM 对脂联素产生的影响。T-MSC CM 是从先前分离和鉴定的 T-MSCs 中收集的，并注射到衰老加速型小鼠 prone 6 中，该小鼠表现出衰老和肥胖的特征。结果表明，注射 T-MSC CM 后，小鼠体重和附睾脂肪组织（eAT）质量减少。与对照组相比，T-MSC CM 注射小鼠的 eAT 中脂联素表达增加，循环中总脂联素和高分子量（HMW）脂联素增加，采用逆转录-定量聚合酶链反应、western blot 分析和 ELISA 检测。在 3T3-L1 脂肪细胞中，T-MSC CM 处理增加了脂联素的分泌和多聚化，这是在非还原和非热变性条件下通过 western blot 检测到的。此外，还使用葡萄糖氧化酶诱导 3T3-L1 脂肪细胞发生氧化应激，观察到 T-MSC CM 减少活性氧的产生和某些氧化应激标志物的表达。此外，结果还表明 HMW 脂联素的产生增加，这表明 T-MSC CM 可能通过改善氧化应激增强脂联素的多聚化。需要进一步研究阐明 T-MSCs 分泌的抗氧化分子，这些结果强调了 T-MSC CM 治疗肥胖或肥胖相关疾病的潜在治疗相关性。
