School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, P.R. China.
Department of Animal Husbandry and Veterinary Medicine, Jiangsu Polytechnic College of Agriculture and Forestry, Jurong, Jiangsu 212499, P.R. China.
Mol Med Rep. 2017 Oct;16(4):5425-5433. doi: 10.3892/mmr.2017.7241. Epub 2017 Aug 14.
In the present study, comparative proteomic analysis was performed in rats subjected to water immersion‑restraint stress (WRS). A total of 26 proteins were differentially expressed and identified using matrix‑assisted laser desorption/ionization time of flight mass spectrometry. Among the 26 differentially expressed protein spots identified, 13 proteins were significantly upregulated under WRS, including pyruvate kinase and calreticulin, which may be closely associated with energy metabolism. In addition, 12 proteins were downregulated under WRS, including hemoglobin subunit β‑2 and keratin type II cytoskeletal 8, which may be important in protein metabolism and cell death. Gene Ontology analysis revealed the cellular distribution, molecular function and biological processes of the identified proteins. The mRNA levels of certain differentially expressed proteins were analyzed using fluorescence quantitative polymerase chain reaction analysis. The results of the present study aimed to offer insights into proteins, which are differentially expressed in gastric ulcers in stress, and provide theoretical evidence of a radical cure for gastric ulcers in humans.
在本研究中，对浸水束缚应激（WRS）大鼠进行了比较蛋白质组学分析。使用基质辅助激光解吸/电离飞行时间质谱法鉴定了 26 种差异表达的蛋白质。在鉴定的 26 个差异表达蛋白斑点中，有 13 种在 WRS 下显著上调，包括丙酮酸激酶和钙网蛋白，它们可能与能量代谢密切相关。此外，WRS 下有 12 种蛋白质下调，包括血红蛋白亚基β-2 和角蛋白 II 细胞骨架 8，它们可能在蛋白质代谢和细胞死亡中起重要作用。基因本体分析揭示了鉴定蛋白的细胞分布、分子功能和生物过程。使用荧光定量聚合酶链反应分析分析了某些差异表达蛋白的 mRNA 水平。本研究旨在深入了解应激性胃溃疡中差异表达的蛋白质，为人类根治胃溃疡提供理论依据。
