Medicinal Chemistry and Pharmacology Institute, Inner Mongolia University for Nationalities, Tongliao, Inner Mongolia 028000, P.R. China.
Oncol Rep. 2017 Oct;38(4):2219-2228. doi: 10.3892/or.2017.5911. Epub 2017 Aug 21.
Triple-negative breast cancer (TNBC) has attracted great attention due to its unique biology, poor prognosis, and aggressiveness. TNBC patients are more likely to suffer from metastasis. We screened and identified the TNBC-specific genes as potential biomarkers. A total of 167 breast cancer samples (45 TNBC and 122 non-TNBC) were used in the integrated analysis. Gene expression microarrays were used to screen the differentially expressed genes. We identified 65 core DEGs. According to the GO and KEGG analysis, the gene function enrichment in TNBC was revealed, such as basal cell carcinoma, prostate cancer, oocyte meiosis and choline metabolism in cancer pathways. Moreover, the PPI network reconstruction would benefit the screening of hubs. A RFS analysis of TNBC-specific genes was also conducted. RT-PCR was used to validate the expression pattern of hubs in TNBC. Finally, nine genes were identified and all of them were novel, specific and higher dysregulation expressed genes in TNBC. Such that, these genes will serve as potential biomarkers in TNBC and benefit further research in TNBC.
三阴性乳腺癌（TNBC）因其独特的生物学特性、不良预后和侵袭性而备受关注。TNBC 患者更容易发生转移。我们筛选并鉴定了 TNBC 特异性基因作为潜在的生物标志物。共使用了 167 例乳腺癌样本（45 例 TNBC 和 122 例非 TNBC）进行综合分析。基因表达微阵列用于筛选差异表达基因。我们鉴定了 65 个核心 DEGs。根据 GO 和 KEGG 分析，揭示了 TNBC 中的基因功能富集，如基底细胞癌、前列腺癌、卵母细胞减数分裂和癌症途径中的胆碱代谢。此外，PPI 网络重构将有助于筛选枢纽。还对 TNBC 特异性基因进行了 RFS 分析。RT-PCR 用于验证枢纽在 TNBC 中的表达模式。最后，确定了九个基因，它们都是 TNBC 中新型、特异性和更高失调表达的基因。因此，这些基因将作为 TNBC 的潜在生物标志物，有助于进一步研究 TNBC。
