Department of Anesthesia, People Hospital of Zoucheng City, Jining, Shandong 272000, P.R. China.
Department of Anesthesia, Weihai Central Hospital, Weihai, Shandong 264400, P.R. China.
Mol Med Rep. 2017 Oct;16(4):5312-5320. doi: 10.3892/mmr.2017.7305. Epub 2017 Aug 21.
Anesthetic agents are used in surgical operations to reversibly reduce consciousness and pain. Sevoflurane is an inhalational anesthetic. Propofol is a short‑acting intravenous general anesthetic. The mechanism of anesthetic agents at pathway level on individual patients has not been reported to date. In the present study, pathway aberrance in the human atrial tissue in response to anesthetics was examined. Microarray data of anesthesia‑treated samples were downloaded from the Array Express database. Pathway information was obtained from the Reactome Pathway Database. The individual pathway aberrance score (iPAS) was introduced to identify dysregulated pathways in individual patients. The present data demonstrated 157 dysregulated pathways in the sevoflurane group, and 44 pathways were identified with the least P‑values. A subset of 49 differentially expressed genes (DEGs) that were shared between the expression profiling results and the dysregulated pathways results were constructed into a co‑expression network. The top 5 ranked DEGs, nuclear receptor subfamily 4 group A member 3 (NR4A3), JUNB proto‑oncogene, MYC proto‑oncogene, tachykinin precursor 1 and nicotinamide phosphoribosyltransferase, were identified as important in the topology analysis. In the propofol group, 87 dysregulated pathways were identified and 44 pathways had the least P‑values. In total 28 DEGs were constructed into a co‑expression network, of which 5 DEGs were important in the topology analysis, NR4A3, suppressor of cytokine signaling 3, cyclin dependent kinase inhibitor 1A, C‑C motif chemokine ligand 2 and C‑X‑C motif chemokine ligand 1. A total of 72 dysregulated pathways were identified in common in the two groups. In conclusion, the two types of anesthetics induced partially similar mechanisms. The pathways enriched by DEGs, particularly those that were unique to sevoflurane and propofol, may affect surgical outcomes and aid the prevention of complications from anesthetics.
麻醉剂用于手术中可逆地降低意识和疼痛。七氟醚是一种吸入性麻醉剂。异丙酚是一种短效静脉全身麻醉剂。迄今为止，尚未报道麻醉剂在个体患者的通路水平上的作用机制。本研究检测了麻醉剂处理的人类心房组织中的通路改变。从 Array Express 数据库下载麻醉处理样本的微阵列数据。从 Reactome 通路数据库获取通路信息。引入个体通路异常评分（iPAS）以识别个体患者中失调的通路。本数据显示七氟醚组有 157 条失调通路，其中 44 条通路的 P 值最小。从表达谱结果和失调通路结果中筛选出一组 49 个差异表达基因（DEG），构建成一个共表达网络。前 5 个排名的 DEG，核受体亚家族 4 组 A 成员 3（NR4A3）、JUNB 原癌基因、MYC 原癌基因、速激肽前体 1 和烟酰胺磷酸核糖基转移酶，在拓扑分析中被认为是重要的。在异丙酚组中，鉴定出 87 条失调通路，其中 44 条通路的 P 值最小。总共构建了 28 个 DEG 共表达网络，其中 5 个 DEG 在拓扑分析中很重要，NR4A3、细胞因子信号转导抑制因子 3、周期蛋白依赖性激酶抑制剂 1A、C-C 基序趋化因子配体 2 和 C-X-C 基序趋化因子配体 1。两组共鉴定出 72 条失调通路。总之，两种类型的麻醉剂诱导了部分相似的机制。DEG 富集的通路，特别是那些七氟醚和异丙酚特有的通路，可能会影响手术结果，并有助于预防麻醉并发症。
