牛磺酸和精氨酸对胰岛素抵抗性高血压大鼠血管平滑肌细胞凋亡的影响。
Effects of Taurine and L-Arginine on the Apoptosis of Vascular Smooth Muscle Cells in Insulin Resistance Hypertensive Rats.
机构信息
Liaoning Provincial Key Laboratory of Zoonoses, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, Liaoning, 110866, People's Republic of China.
出版信息
Adv Exp Med Biol. 2017;975 Pt 2:813-819. doi: 10.1007/978-94-024-1079-2_63.
OBJECTIVE
In order to find the effects and mechanism of taurine and L-arginine on the apoptosis of VSMCs in insulin resistance hypertensive rats.
METHODS
25% fructose were administered in the drinking water to Wistar rats for 12 weeks to induce the insulin resistance hypertensive model. Apoptosis of VSMCs was identified by TUNEL. The expressions of Bax protein and Bcl-2 protein were examined by immunohistochemistry.
RESULTS
High level of fructose significantly suppressed the apoptosis of VSMCs. Taurine and L-arginine promoted apoptosis of VSMCs via increasing the Bax protein expression and decreasing the Bcl-2 protein expression.
CONCLUSIONS
Taurine and L-arginine have obvious anti-hypertensive effects in insulin resistance hypertensive rats, and its mechanism might partially be associated with attenuating vascular remodeling by promoting apoptosis in VSMCs. In addition, the combined medication of taurine and L-arginine was more effective than single medication.
目的
探讨牛磺酸和精氨酸对胰岛素抵抗性高血压大鼠血管平滑肌细胞凋亡的作用及机制。
方法
采用 25%果糖饮水喂养 Wistar 大鼠 12 周建立胰岛素抵抗性高血压模型，TUNEL 法检测血管平滑肌细胞凋亡，免疫组化法检测 Bax 蛋白、Bcl-2 蛋白表达。
结果
高浓度果糖明显抑制血管平滑肌细胞凋亡，牛磺酸和精氨酸通过增加 Bax 蛋白表达、减少 Bcl-2 蛋白表达促进血管平滑肌细胞凋亡。
结论
牛磺酸和精氨酸对胰岛素抵抗性高血压大鼠具有明显的降压作用，其机制可能与促进血管平滑肌细胞凋亡、减轻血管重构有关。且两者联合用药的效果优于单药。
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