Hirota K, Hirota T, Sanno Y, Tanaka T
Cancer Res. 1987 Jul 15;47(14):3742-6.
Previously a new glucocorticoid receptor (Peak C), which eluted with 0.12 to 0.14 M NaCl from DEAE-cellulose column, was identified in addition to another receptor (Peak B), a classic type of glucocorticoid receptor, which eluted with 0.05 to 0.08 M NaCl. Peak C appeared after stress or injection of a high dose (20 micrograms/100 g body weight) of dexamethasone into rats. Peak C was also detected in the liver of rats bearing various tumors, but it was not found in malignant tumors (Yoshida sarcoma and Yoshida ascites hepatoma AH 130), a less malignant Yoshida ascites hepatoma (LY-5), or in minimal deviation-type hepatomas (Morris hepatomas 7316A and 7794A). The absence of Peak C in these tumors coincided with the inability of the glucocorticoid to induce tryptophan oxygenase in these tumors and in the liver of rats during early postnatal development. Peak B was consistently observed in various hepatomas and immature rat liver with capability to induce tyrosine aminotransferase. Thus Peak C appeared to be a highly differentiated type of glucocorticoid receptor mediating specific hormone actions and to be present in mature liver cells, but not in immature liver or tumor cells, even of the minimal deviation type.
以前，除了另一种受体（峰B），即一种经典类型的糖皮质激素受体，在0.05至0.08M NaCl条件下从DEAE - 纤维素柱上洗脱下来之外，还鉴定出了一种新的糖皮质激素受体（峰C），它在0.12至0.14M NaCl条件下从DEAE - 纤维素柱上洗脱下来。峰C在应激后或向大鼠注射高剂量（20微克/100克体重）地塞米松后出现。在患有各种肿瘤的大鼠肝脏中也检测到了峰C，但在恶性肿瘤（吉田肉瘤和吉田腹水肝癌AH 130）、恶性程度较低的吉田腹水肝癌（LY - 5）或微小偏离型肝癌（莫里斯肝癌7316A和7794A）中未发现。这些肿瘤中峰C的缺失与糖皮质激素在这些肿瘤以及出生后早期发育阶段大鼠肝脏中诱导色氨酸加氧酶的能力缺失相一致。在各种肝癌和具有诱导酪氨酸转氨酶能力的未成熟大鼠肝脏中始终观察到峰B。因此，峰C似乎是一种高度分化的糖皮质激素受体，介导特定的激素作用，存在于成熟肝细胞中，但不存在于未成熟肝脏或肿瘤细胞中，即使是微小偏离型的肿瘤细胞。
