Institute of Hematology, Changhai Hospital, Second Military Medical University, Shanghai, 200433, China.
Department of Hematology, Shanghai First People's Hospital, Shanghai Jiaotong University, Shanghai, 200433, China.
J Transl Med. 2017 Aug 29;15(1):179. doi: 10.1186/s12967-017-1287-4.
High expression of proviral integration site for Moloney murine leukemia virus-1 (PIM-1), a serine/threonine kinase, is associated with many cancers. The main purpose of this study were to investigate that the correlation between PIM-1 mRNA levels and clinicopathologic features and its clinical significance in acute myeloid leukemia (AML).
qRT-PCR was performed for 118 de novo AML and 20 AML complete remission patients and 15 normal individuals. All statistical analysis were performed using Graphpad Prism5 software.
We observed that expression of PIM-1 mRNA was higher in AML patients than in healthy individuals and in complete remission AML patients (P = 0.0177). Further, high PIM-1 mRNA levels were more associated with high-risk FLT3+ AML patients than the FLT3- group (P = 0.0001) and were also associated with clinical factors such as risk stratification (P = 0.0029) and vital status (P = 0.0322). Kaplan-Meier survival analysis indicated that PIM-1 mRNA expression correlated with overall survival (OS), disease free survival (DFS), and relapse rate (RR) in AML patients. Most importantly, the high PIM-1-expressing patients took longer to achieve complete remission than the low expression group (P = 0.001). In addition, the complete remission rate was significantly lower in the high PIM-1 group (P = 0.0277) after induction therapy.
Above results suggest that PIM-1 mRNA levels may be an independent prognostic factor in AML.
原癌基因整合位点 Moloney 鼠白血病病毒-1（PIM-1）是一种丝氨酸/苏氨酸激酶，其高表达与许多癌症相关。本研究的主要目的是探讨 PIM-1mRNA 水平与临床病理特征的相关性及其在急性髓系白血病（AML）中的临床意义。
采用 qRT-PCR 检测 118 例初诊 AML 患者、20 例 AML 完全缓解患者和 15 例正常人的 PIM-1mRNA 水平。所有统计分析均采用 Graphpad Prism5 软件进行。
我们观察到 AML 患者的 PIM-1mRNA 表达高于健康个体和完全缓解 AML 患者（P=0.0177）。进一步研究发现，高 PIM-1mRNA 水平与 FLT3+高风险 AML 患者更为相关，而与 FLT3-组无显著相关性（P=0.0001），并且与临床因素如风险分层（P=0.0029）和生存状态（P=0.0322）相关。Kaplan-Meier 生存分析表明，PIM-1mRNA 表达与 AML 患者的总生存（OS）、无病生存（DFS）和复发率（RR）相关。更为重要的是，高 PIM-1 表达组患者达到完全缓解的时间长于低表达组（P=0.001）。此外，在诱导治疗后，高 PIM-1 组的完全缓解率明显低于低表达组（P=0.0277）。
上述结果表明，PIM-1mRNA 水平可能是 AML 的一个独立预后因素。
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