Sherbany A A, Parent A S, Brosius J
DNA. 1987 Jun;6(3):267-72. doi: 10.1089/dna.1987.6.267.
We report the isolation of a clone encoding calmodulin from a rat brain cDNA library; genomic clones were also isolated and partially characterized. The derived amino acid sequence has 100% homology to the published mammalian protein sequences. In rat, we identify two major RNA species of approximately 2.2 and 0.75 kb in length by RNA blot analysis. Furthermore, the mRNA is highly enriched in brain tissue, compared to liver or kidney. A variant (GATAAA) of the putative poly(A) addition signal is observed at the 3' end of one of the mRNA species.
我们报道了从大鼠脑cDNA文库中分离出一个编码钙调蛋白的克隆；同时也分离出了基因组克隆并对其进行了部分特征分析。推导的氨基酸序列与已发表的哺乳动物蛋白质序列具有100%的同源性。在大鼠中，通过RNA印迹分析，我们鉴定出两种主要的RNA种类，长度分别约为2.2 kb和0.75 kb。此外，与肝脏或肾脏相比，该mRNA在脑组织中高度富集。在其中一种mRNA种类的3'端观察到推定的聚腺苷酸化信号的一个变体（GATAAA）。
