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SDE5，一种假定的 RNA 输出蛋白，通过拟南芥中鞭毛蛋白依赖的信号通路参与植物先天免疫。

SDE5, a putative RNA export protein, participates in plant innate immunity through a flagellin-dependent signaling pathway in Arabidopsis.

机构信息

College of Pharmacy and Research Institute of Pharmaceutical Science, PMBBRC, Gyeongsang National University, Jinju, 660-701, Republic of Korea.

Plant Breeding Institute, School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Narrabri, NSW, 2390, Australia.

出版信息

Sci Rep. 2017 Aug 29;7(1):9859. doi: 10.1038/s41598-017-07918-x.

DOI:10.1038/s41598-017-07918-x
PMID:28851870
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5574965/
Abstract

In eukaryotes, RNA silencing, mediated by small interfering RNAs, is an evolutionarily widespread and versatile silencing mechanism that plays an important role in various biological processes. Increasing evidences suggest that various components of RNA silencing pathway are involved in plant defense machinery against microbial pathogens in Arabidopsis thaliana. Here, we show genetic and molecular evidence that Arabidopsis SDE5 is required to generate an effective resistance against the biotrophic bacteria Pseudomonas syringae pv. tomato DC3000 and for susceptibility to the necrotrophic bacteria Erwinia caratovora pv. caratovora. SDE5, encodes a putative mRNA export factor that is indispensable for transgene silencing and the production of trans-acting siRNAs. SDE5 expression is rapidly induced by exogenous application of phytohormone salicylic acid (SA), methyl jasmonate (MeJA), phytopathogenic bacteria, and flagellin. We further report that SDE5 is involved in basal plant defense and mRNA export. Our genetic data suggests that SDE5 and Nonexpressor of PR Gene1 (NPR1) may contribute to the same SA-signaling pathway. However, SDE5 over-expressing transgenic plant exhibits reduced defense responsive phenotype after flagellin treatment. Taken together, these results support the conclusion that SDE5 contributes to plant innate immunity in Arabidopsis.

摘要

在真核生物中，由小干扰 RNA 介导的 RNA 沉默是一种进化上广泛存在且多功能的沉默机制，在各种生物过程中发挥着重要作用。越来越多的证据表明，RNA 沉默途径的各种成分参与了拟南芥中植物防御机制对微生物病原体的防御。在这里，我们通过遗传和分子证据表明，拟南芥 SDE5 是对生细菌丁香假单胞菌 pv.番茄 DC3000 产生有效抗性所必需的，并且对坏死细菌胡萝卜软腐欧文氏菌 pv.胡萝卜软腐菌敏感。SDE5 编码一种假定的 mRNA 输出因子，对于转基因沉默和反式作用 siRNA 的产生是必不可少的。SDE5 的表达被外源施加的植物激素水杨酸（SA）、茉莉酸甲酯（MeJA）、植物病原菌和鞭毛蛋白迅速诱导。我们进一步报告 SDE5 参与基础植物防御和 mRNA 输出。我们的遗传数据表明，SDE5 和 Nonexpressor of PR Gene1（NPR1）可能参与相同的 SA 信号通路。然而，在 flagellin 处理后，SDE5 过表达转基因植物表现出防御反应表型的降低。综上所述，这些结果支持 SDE5 有助于拟南芥植物先天免疫的结论。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ae5/5574965/abd7feb7716d/41598_2017_7918_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ae5/5574965/204023540437/41598_2017_7918_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ae5/5574965/b22df6f70521/41598_2017_7918_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ae5/5574965/c2013ebc0ce6/41598_2017_7918_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ae5/5574965/90dd3ce8f554/41598_2017_7918_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ae5/5574965/3e9aff80e1ff/41598_2017_7918_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ae5/5574965/15f009bdc693/41598_2017_7918_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ae5/5574965/e99756c7a6aa/41598_2017_7918_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ae5/5574965/abd7feb7716d/41598_2017_7918_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ae5/5574965/204023540437/41598_2017_7918_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ae5/5574965/b22df6f70521/41598_2017_7918_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ae5/5574965/c2013ebc0ce6/41598_2017_7918_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ae5/5574965/90dd3ce8f554/41598_2017_7918_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ae5/5574965/3e9aff80e1ff/41598_2017_7918_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ae5/5574965/15f009bdc693/41598_2017_7918_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ae5/5574965/e99756c7a6aa/41598_2017_7918_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ae5/5574965/abd7feb7716d/41598_2017_7918_Fig8_HTML.jpg

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