Peking University People's Hospital, Beijing, China.
The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
Sci Rep. 2017 Aug 29;7(1):9818. doi: 10.1038/s41598-017-10127-1.
After Peripheral nerve injuries (PNI), many complicated pathophysiologic processes will happen. A global view of functional changes following PNI is essential for the looking for the adequate therapeutic approaches. In this study, we performed an in-depth analysis on the temporal expression profiles after sciatic nerve injury by bioinformatic methods, including (1) cluster analysis of the samples; (2) identification of gene co-expression modules(CEMs) correlated with the time points; (3) analysis of differentially expressed genes at each time point (DEGs-ET); (4) analysis of differentially expressed genes varying over time (DEGs-OT); (5) creating Pairwise Correlation Plot for the samples; (6) Time Series Regression Analysis; (7) Determining the pathway, GO (gene ontology) and drug by enrichment analysis. We found that at a 3 h "window period" some specific gene expression may exist after PNI, and responses to lipopolysaccharide (LPS) and TNF signaling pathway may play important roles, suggesting that the inflammatory microenvironment exists after PNI. We also found that troglitazone was closely associated with the change of gene expression after PNI. Therefore, the further evaluation of the precise mechanism of troglitazone on PNI is needed and it may contribute to the development of new drugs for patients with PNI.
周围神经损伤（PNI）后，会发生许多复杂的病理生理过程。了解 PNI 后功能变化的全貌对于寻找合适的治疗方法至关重要。在这项研究中，我们通过生物信息学方法对坐骨神经损伤后的时间表达谱进行了深入分析，包括（1）样本聚类分析；（2）鉴定与时间点相关的基因共表达模块（CEMs）；（3）分析每个时间点的差异表达基因（DEGs-ET）；（4）分析随时间变化的差异表达基因（DEGs-OT）；（5）为样本创建成对相关图；（6）时间序列回归分析；（7）通过富集分析确定途径、GO（基因本体论）和药物。我们发现，在 PNI 后的 3 小时“窗口期”内，可能存在一些特定的基因表达，脂多糖（LPS）和 TNF 信号通路的反应可能起着重要作用，表明 PNI 后存在炎症微环境。我们还发现曲格列酮与 PNI 后基因表达的变化密切相关。因此，需要进一步评估曲格列酮对 PNI 的精确作用机制，这可能有助于开发治疗 PNI 患者的新药。
