Manson M M, Neal G E
Food Addit Contam. 1987 Apr-Jun;4(2):141-7. doi: 10.1080/02652038709373625.
The detoxification of aflatoxin-containing groundnut meal by ammoniation has been investigated using weanling male and female Fischer 344 rats. Rats were fed a pelleted diet consisting of 75% groundnut meal and 25% powdered rat diet, with vitamin supplement, for a period of 90 days. The groundnut meal was either aflatoxin-free or naturally contaminated with aflatoxins, and was used untreated or after ammoniation. Body weights and food consumptions were monitored throughout the experimental feeding period. After 90 days the animals were sacrificed and the gamma-glutamyl transferase (GGT) levels in the livers assayed fluorimetrically, and the distribution of the enzyme activity determined histochemically. The results indicated that ammoniation of the aflatoxin-containing meal eliminated the development of focal GGT positive lesions in both male and female animals. However, in the female, histochemical GGT staining of hepatocytes in the periportal areas was increased by all the experimental diets compared with the unammoniated, aflatoxin-free diet. This observation was supported by the fluorimetric assays. Ammoniation of the diet led to a decreased body weight gain in all cases compared with the corresponding unammoniated diet-fed groups.
利用断乳的雄性和雌性Fischer 344大鼠，研究了通过氨化作用对含黄曲霉毒素的花生粕进行解毒。给大鼠喂食一种颗粒饲料，该饲料由75%的花生粕、25%的粉状大鼠饲料以及维生素补充剂组成，持续90天。花生粕要么不含黄曲霉毒素，要么天然受到黄曲霉毒素污染，且未处理或经过氨化处理后使用。在整个实验喂养期间监测体重和食物消耗量。90天后处死动物，用荧光法测定肝脏中的γ-谷氨酰转移酶（GGT）水平，并用组织化学方法确定酶活性的分布。结果表明，对含黄曲霉毒素的饲料进行氨化处理可消除雄性和雌性动物中局灶性GGT阳性病变的发展。然而，在雌性动物中，与未经氨化处理的无黄曲霉毒素饲料相比，所有实验饲料都增加了门周区域肝细胞的组织化学GGT染色。荧光测定法支持了这一观察结果。与相应的未氨化饲料喂养组相比，饲料氨化处理在所有情况下均导致体重增加减少。
