培养的3T3-L1脂肪细胞中谷氨酰胺合成酶基因转录：地塞米松、胰岛素和二丁酰环磷腺苷的调节作用

Glutamine synthetase gene transcription in cultured 3T3-L1 adipocytes: regulation by dexamethasone, insulin and dibutyryl cyclic AMP.

作者信息

Bhandari B, Miller R E

出版信息

Mol Cell Endocrinol. 1987 May;51(1-2):7-11. doi: 10.1016/0303-7207(87)90112-2.

Abstract

We have investigated the regulation of glutamine synthetase (GS) mRNA synthesis in cultured 3T3-L1 adipocytes. Specific mRNA synthesis (transcription) was analyzed by measuring elongation of transcripts in isolated nuclei. Transcription rate was assayed by hybridization of newly synthesized [32P]RNA to a GS cDNA. GS transcription rate increased more than 100-fold during adipocyte differentiation and was inhibited more than 90% by alpha-amanitin. In 3T3-L1 adipocytes dexamethasone stimulated GS gene transcription while insulin and dibutyryl cAMP decreased GS gene transcription.

摘要

我们研究了培养的3T3-L1脂肪细胞中谷氨酰胺合成酶（GS）mRNA合成的调控。通过测量分离细胞核中转录本的延伸来分析特异性mRNA合成（转录）。转录速率通过将新合成的[32P]RNA与GS cDNA杂交来测定。在脂肪细胞分化过程中，GS转录速率增加了100多倍，并且被α-鹅膏蕈碱抑制了90%以上。在3T3-L1脂肪细胞中，地塞米松刺激GS基因转录，而胰岛素和二丁酰环磷腺苷降低GS基因转录。