Gomez-Flores Ricardo, Espinosa-Ramos David, Quintanilla-Licea Ramiro, Barrón-Gonzalez María Porfiria, Tamez-Guerra Patricia, Tamez-Guerra Reyes, Rodriguez-Padilla Cristina
Departamento de Química, Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Departamento de Microbiología e Inmunología.
Departamento de Biología Celular y Genética.
Afr J Tradit Complement Altern Med. 2016 Jul 3;13(4):55-59. doi: 10.21010/ajtcam.v13i4.9. eCollection 2016.
Prolonged use of antibiotics may lead to the selection of drug-resistant bacteria; as a result, efforts are being made to identify new and effective antimicrobial agents, particularly, from medicinal plants, against bacterial infections. Antimicrobial activity of against has not yet been reported.
The antibacterial effect of methanol leaf extracts against (ATCC 43504) was evaluated in liquid medium by the colorimetric 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) reduction assay and in solid medium by the colony forming units (CFU) method.
Methanol extracts significantly (<0.05) inhibited growth in liquid medium from 24% to 82% at concentrations ranging from 31.25 mg/ml to 500 mg/ml, respectively, and in solid medium the extracts caused significant (<0.05) 52% and 100% bacterial growth inhibition at concentrations of 250 μg/mL and 500 μg/mL, respectively, as compared with untreated control. Methanol vehicle did not affect growth.
The observed antibacterial effect of extracts may be of benefit as an adjuvant treatment of diseases caused by .
长期使用抗生素可能导致耐药菌的产生；因此，人们正在努力寻找新的、有效的抗菌剂，特别是从药用植物中寻找对抗细菌感染的抗菌剂。关于[植物名称]对[细菌名称]的抗菌活性尚未见报道。
通过比色法3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴盐（MTT）还原试验在液体培养基中以及通过菌落形成单位（CFU）法在固体培养基中评估[植物名称]甲醇叶提取物对[细菌名称]（ATCC 43504）的抗菌效果。
甲醇提取物在浓度范围为31.25毫克/毫升至500毫克/毫升时，分别在液体培养基中显著（<0.05）抑制[细菌名称]生长24%至82%，在固体培养基中，与未处理的对照相比，提取物在浓度为250微克/毫升和500微克/毫升时分别导致显著（<0.05）的52%和100%的细菌生长抑制。甲醇载体不影响[细菌名称]生长。
观察到的[植物名称]提取物的抗菌作用可能有助于作为由[细菌名称]引起的疾病的辅助治疗。
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