Masadeh Majed M, Alkofahi Ahmad S, Alzoubi Karem H, Tumah Haitham N, Bani-Hani Kamal
Department of Pharmaceutical Technology .
Pharm Biol. 2014 May;52(5):566-9. doi: 10.3109/13880209.2013.853811. Epub 2013 Nov 20.
Natural flora are considered a major source of new agents for the treatment of Helicobactor pylori. The plants used in this study were selected based on previous traditional use.
In this study, we evaluated the effect of extracts of 16 medicinal plants grown in Jordan against clinical isolates of H. pylori.
Tested plant extracts included Aloysia triphylla (L'Her.) Britton (Verbenaceae), Anethum graveolens L. (Apiaceae), Artemisia inculata Delile (Asteraceae), Capparis spinosa L. (Capparaceae), Crataegus aronia (L.) Bosc ex. DC. (Rosaceae), Inula viscose (L.) Ait (Asteraceae), Lavandula officinalis Chaix. (Lamiaceae), Lepidium sativum L. (Cruciferae), Origanum syriaca L. (Lamiaceae), Paronychia argentea Lam. (Caryophyllaceae), Passiflora incarnate L. (Passifloraceae), Psidium guajava L. (Myrtaceae), Sarcopoterium spinosum (L.) Spach (Rosaceae), Sesamum indicum L. (Pedaliaceae), Urtica urens L. (Urticaceae) and Varthemia iphionoids Boiss (Asteraceae). Clinical isolates of H. pylori were tested in vitro for susceptibility to each of the above plant crude extracts using disk diffusion method, and the MIC value was determined for each plant extract using the serial dilution method.
Results showed that ethanol extracts of most medicinal plants exerted cytotoxiciy against H. pylori isolates. Among the tested plant extracts, A. triphylla (MIC: 90 µg/mL, MBC: 125 µg/mL) and I. viscosa (MIC: 83 µg/mL, MBC: 104 µg/mL) showed the strongest activity against both isolates of H. pylori.
Jordanian medicinal plants might be valuable sources of starting materials for the synthesis of new antibacterial agents against H. pylori.
自然菌群被认为是治疗幽门螺杆菌新药物的主要来源。本研究中使用的植物是根据以往的传统用途挑选出来的。
在本研究中，我们评估了约旦种植的16种药用植物提取物对幽门螺杆菌临床分离株的作用。
受试植物提取物包括三角叶香茶菜（唇形科）、莳萝（伞形科）、埃及蒿（菊科）、刺山柑（山柑科）、山楂（蔷薇科）、粘旋覆花（菊科）、薰衣草（唇形科）、独行菜（十字花科）、叙利亚牛至（唇形科）、银指甲草（石竹科）、西番莲（西番莲科）、番石榴（桃金娘科）、多刺蔷薇（蔷薇科）、芝麻（胡麻科）、小荨麻（荨麻科）和岩生帚菊（菊科）。采用纸片扩散法体外检测幽门螺杆菌临床分离株对上述每种植物粗提取物的敏感性，采用系列稀释法测定每种植物提取物的最低抑菌浓度（MIC）值。
结果表明，大多数药用植物的乙醇提取物对幽门螺杆菌分离株具有细胞毒性。在受试植物提取物中，三角叶香茶菜（MIC：90μg/mL，MBC：125μg/mL）和粘旋覆花（MIC：83μg/mL，MBC：104μg/mL）对两种幽门螺杆菌分离株均表现出最强的活性。
约旦药用植物可能是合成抗幽门螺杆菌新抗菌剂的有价值原料来源。
