番茄红素对人胃癌细胞生长的抑制作用
INHIBITORY EFFECT OF LYCOPENE AGAINST THE GROWTH OF HUMAN GASTRIC CANCER CELLS.
作者信息
Zhou ShenKang, Zhang RuiLi, Bi TieNan, Lu Yong, Jiang LiangXian
机构信息
Department of Gastrointestinal surgery, Taizhou hospital, TaiZhou city, Zhejiang, china.
出版信息
Afr J Tradit Complement Altern Med. 2016 Jul 3;13(4):184-190. doi: 10.21010/ajtcam.v13i4.24. eCollection 2016.
BACKGROUND
The aim of this study was to investigate the anti-proliferative effect of Lycopene on HGC-27 cells.
MATERIALS AND METHODS
HGC-27 cells were treated with varying concentration lycopene for 24, 48, 72 h. The cell growth inhibition was analyzed by MTT. Western blotting was used to indicate changes in the levels of LC3-I, LC3-II, ERK (extracellular signal-regulated protein kinase) and phosphorylation-ERK (p-ERK).
RESULTS
Lycopene displayed antiproliferative activity in HGC-27 cell lines. Western blotting showed that Lycopene significantly enhanced LC3-I, p-ERK proteins expression. In gastric cancer nude mice model, lycopene treatment significantly decreased tumour weight. These findings indicated that lycopene treatment induces the anti-proliferation of HGC-27 cells.
CONCLUSION
Lycopene treatment inhibited HGC-27 cells growth by activating ERK.
背景
本研究旨在探讨番茄红素对HGC - 27细胞的抗增殖作用。
材料与方法
用不同浓度的番茄红素处理HGC - 27细胞24、48、72小时。通过MTT分析细胞生长抑制情况。采用蛋白质免疫印迹法检测LC3 - I、LC3 - II、细胞外信号调节蛋白激酶（ERK）及磷酸化细胞外信号调节蛋白激酶（p - ERK）水平的变化。
结果
番茄红素在HGC - 27细胞系中表现出抗增殖活性。蛋白质免疫印迹法显示，番茄红素显著增强了LC3 - I、p - ERK蛋白的表达。在胃癌裸鼠模型中，番茄红素治疗显著降低了肿瘤重量。这些结果表明，番茄红素治疗可诱导HGC - 27细胞的抗增殖作用。
结论
番茄红素通过激活ERK抑制HGC - 27细胞生长。
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