Romanov Yu A, Volgina N E, Balashova E E, Kabaeva N V, Dugina T N, Sukhikh G T
Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia.
CryoCenter Cord Blood Bank, Moscow, Russia.
Bull Exp Biol Med. 2017 Aug;163(4):523-527. doi: 10.1007/s10517-017-3843-6. Epub 2017 Aug 29.
Cell-cell interactions and the ability of mesenchymal stromal cells to support the expansion of hematopoietic progenitor cells were studied in co-culture of human umbilical cord tissue-derived mesenchymal stromal cells and nucleated umbilical cord blood cells. It was found that hematopoietic stem cells from the umbilical cord blood are capable to adhere to mesenchymal stromal cells and proliferate during 3-4 weeks in co-culture. However, despite the formation of hematopoietic foci and accumulation of CD34 and CD133 cells in the adherent cell fraction, the ability of newly generated blood cells to form colonies in semi-solid culture medium was appreciably reduced. These findings suggest that human umbilical cord tissue-derived mesenchymal stromal cells display a weak capability to support the "stemness" of hematopoietic stem cell progeny despite long-term maintenance of their viability and proliferation.
在人脐带组织来源的间充质基质细胞与有核脐带血细胞的共培养中，研究了细胞间相互作用以及间充质基质细胞支持造血祖细胞扩增的能力。结果发现，脐带血中的造血干细胞能够黏附于间充质基质细胞，并在共培养3 - 4周期间增殖。然而，尽管在贴壁细胞部分形成了造血灶以及CD34和CD133细胞积累，但新生成的血细胞在半固体培养基中形成集落的能力明显降低。这些发现表明，人脐带组织来源的间充质基质细胞尽管能长期维持造血干细胞后代的活力和增殖，但其支持造血干细胞后代“干性”的能力较弱。
