Duan Xinchong, Li Yang, Di Keqian, Huang Yong, Li Xiangyun
College of Animal Science and Technology, Hebei Agricultural University, Baoding 071001, Hebei, China.
Centre of Laboratory Animal Faculty of Medicine, Hebei University, Baoding 071001, Hebei, China.
Sheng Wu Gong Cheng Xue Bao. 2016 Apr 25;32(4):440-446. doi: 10.13345/j.cjb.150333.
Although efficient nonsurgical transfer of embryos in mice would provide many advantages over a surgical method, the low success rate of nonsurgical transfer has hampered its acceptance and use. Here, a plastic catheter was used to mimic embryo transfer process and then the transfer efficiency was evaluated by intrauterine trypan blue dye dispersion. Also 3.5-day blastocysts from natural pregnant mice were transferred through cervix into uterine horns. The results show that 70.9% of CD-1 mouse 3.5-day blastocysts transferred into unilateral uterine horns of pseudopregnant 2.5-day recipients can be developed to live newborns, and an efficient mouse nonsurgical embryo transfer technique was established. The technique was simple, rapid, inexpensive, unlikely to get contaminated, ethical and do not need specialized apparatus, and can completely replace surgical embryo transfer techniques. Moreover, the mouse nonsurgical embryo transfer technique provides a research model for human and other large animal embryo transfer.
尽管与手术方法相比，在小鼠中高效的非手术胚胎移植具有诸多优势，但非手术移植的低成功率阻碍了其被接受和使用。在此，使用一根塑料导管模拟胚胎移植过程，然后通过子宫内台盼蓝染料扩散来评估移植效率。此外，将来自自然怀孕小鼠的3.5天囊胚经子宫颈转移至子宫角。结果显示，转移至2.5天假孕受体单侧子宫角的CD-1小鼠3.5天囊胚中有70.9%可发育为存活的新生小鼠，从而建立了一种高效的小鼠非手术胚胎移植技术。该技术简单、快速、廉价，不易污染，符合伦理且无需专门设备，能够完全替代手术胚胎移植技术。此外，小鼠非手术胚胎移植技术为人类和其他大型动物的胚胎移植提供了一个研究模型。
