Ying Wang, Qimin Wang, Jinghua Li, Jinhong Han, Lili Wang, Chen Chao, Jianhua Zhou, Lei Tong, Xufei Lu, Yuan Zhou, Yixiang Liao, Zongxuan He, Ning Li, Lei Cao, Wenjun Liu, Zhenggang Chen
College of Stomatology, Weifang Medical University, Weifang 261021, China.
Dept. of Oral and Maxillofacial Surgery, Qingdao Municipal Hospital, Qingdao 266071, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2017 Aug 1;35(4):373-378. doi: 10.7518/hxkq.2017.04.006.
Objective This study aims to investigate the effect of geranylgeranyltransferaseⅠ (GGTase-Ⅰ) on the proliferation and growth of tongue squamous cancer cells. Methods Three small interfering RNAs (siRNAs) were designed on the basis of the GGTase-Ⅰ sequence in GeneBank. These siRNAs were then transfected into tongue squamous cancer cells Cal-27. The mRNA and protein expression of GGTase-Ⅰ and RhoA were examined by real-time quantitative polymerase chain reaction and Western blotting, respectively. The expression of Cyclin D1 and p21 were examined by Western blotting. The proliferation and growth ability were analyzed by cell counting kit-8 assay and flow cytometry. Results The mRNA and protein expression of GGTase-Ⅰ in Cal-27 was reduced significantly after the GGTase-Ⅰ siRNAs were transfected (P<0.05). No significant difference in RhoA mRNA and protein expression was detected (P>0.05). Cyclin D1 expression decreased, whereas p21 expression increased significantly. The cell cycle was altered, and the growth-proliferative activity was inhibited (P<0.05). Conclusion GGTase-Ⅰ siRNA can inhibit the expression of GGTase-Ⅰ and the proliferative activity of tongue squamous cancer cells. GGTase-Ⅰ may be a potential target for gene therapy in tongue squamous cell cancer.
目的 本研究旨在探讨香叶基香叶基转移酶Ⅰ（GGTase-Ⅰ）对舌鳞状癌细胞增殖和生长的影响。方法 根据基因库中GGTase-Ⅰ序列设计3条小干扰RNA（siRNA）。将这些siRNA转染至舌鳞状癌细胞Cal-27。分别采用实时定量聚合酶链反应和蛋白质印迹法检测GGTase-Ⅰ和RhoA的mRNA及蛋白质表达。采用蛋白质印迹法检测细胞周期蛋白D1（Cyclin D1）和p21的表达。采用细胞计数试剂盒-8法和流式细胞术分析细胞的增殖和生长能力。结果 转染GGTase-Ⅰ siRNA后，Cal-27细胞中GGTase-Ⅰ的mRNA和蛋白质表达显著降低（P<0.05）。RhoA的mRNA和蛋白质表达未检测到显著差异（P>0.05）。Cyclin D1表达降低，而p21表达显著增加。细胞周期发生改变，生长增殖活性受到抑制（P<0.05）。结论 GGTase-Ⅰ siRNA可抑制GGTase-Ⅰ的表达及舌鳞状癌细胞的增殖活性。GGTase-Ⅰ可能是舌鳞状细胞癌基因治疗的潜在靶点。
