Marker Laura Meier, Hammer Anne Sofie, Andresen Lars, Isaack Pernille, Clausen Tove, Byskov Kevin, Honoré Oliver Lykke, Jensen Søren Krogh, Bahl Martin Iain
Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, DK-1870 Frederiksberg C, Denmark.
Danish Fur Breeders Research Centre, DK-7500 Holstebro, Denmark.
FEMS Microbiol Ecol. 2017 Jul 1;93(7). doi: 10.1093/femsec/fix092.
It is well documented that antibiotics have pronounced modulatory effects on the intestinal bacterial community of both humans and animals, with potential health consequences. The gut microbiota of mink has however attracted little attention due to low bacterial load and fast gastrointestinal transit time, questioning its relevance. In this study, we hypothesise that oral amoxicillin treatment affects the gut microbiota in mink. This was investigated in a controlled trial including 24 animals of which 12 were treated with amoxicillin for 7 days. By applying 16S rRNA gene sequencing, we found that the faecal microbiota was markedly altered already after 2 days of treatment, with a surprising increase in diversity to resemble the feed. The diversity within the mucosa at termination was however reduced, which indicates this compartment as an important colonisation site in mink. No impact on blood biochemistry, lipid metabolism, serum amyloid A, vitamins A and E and histomorphology of the gut and liver was found; however, a slight decrease in fat digestibility was observed. We suggest that early-life use of amoxicillin in mink production may be counteractive as dysbiosis of the microbiota during infancy is increasingly being recognised as a risk factor for future health.
抗生素对人类和动物的肠道细菌群落具有显著的调节作用，且存在潜在的健康影响，这一点已有充分记录。然而，由于水貂肠道细菌载量低且胃肠转运时间快，其肠道微生物群很少受到关注，其相关性也受到质疑。在本研究中，我们假设口服阿莫西林治疗会影响水貂的肠道微生物群。在一项对照试验中对这一假设进行了研究，该试验包括24只动物，其中12只接受了7天的阿莫西林治疗。通过应用16S rRNA基因测序，我们发现治疗2天后粪便微生物群就已发生显著改变，多样性惊人地增加，类似于饲料。然而，在试验结束时，黏膜内的多样性降低，这表明该区域是水貂重要的定植部位。未发现对血液生化、脂质代谢、血清淀粉样蛋白A、维生素A和E以及肠道和肝脏组织形态学有影响；然而，观察到脂肪消化率略有下降。我们认为，在水貂生产中早期使用阿莫西林可能会适得其反，因为婴儿期微生物群失调越来越被认为是未来健康的一个风险因素。
