Santos Katz I S, Dias M H, Lima I F, Chaves L B, Ribeiro O G, Scheffer K C, Iwai L K
Acta Virol. 2017;61(3):280-288. doi: 10.4149/av_2017_306.
Rabies is a zoonotic viral disease that remains a serious threat to public health worldwide. The rabies lyssavirus (RABV) genome encodes five structural proteins, multifunctional and significant for pathogenicity. The large protein (L) presents well-conserved genomic regions, which may be a good alternative to generate informative datasets for development of new methods for rabies diagnosis. This paper describes the development of a technique for the identification of L protein in several RABV strains from different hosts, demonstrating that MS-based proteomics is a potential method for antigen identification and a good alternative for rabies diagnosis.
狂犬病是一种人畜共患的病毒性疾病，仍然对全球公共卫生构成严重威胁。狂犬病病毒（RABV）基因组编码五种结构蛋白，这些蛋白具有多功能且对致病性具有重要意义。大蛋白（L）呈现出高度保守的基因组区域，这可能是生成信息数据集以开发狂犬病诊断新方法的良好选择。本文描述了一种用于鉴定来自不同宿主的几种RABV毒株中L蛋白的技术的开发，表明基于质谱的蛋白质组学是一种潜在的抗原鉴定方法，也是狂犬病诊断的良好选择。
