Nirmala Jayaveeramuthu, Saini Jyoti, Newcomb Maria, Olivera Pablo, Gale Sam, Klindworth Daryl, Elias Elias, Talbert Luther, Chao Shiaoman, Faris Justin, Xu Steven, Jin Yue, Rouse Matthew N
Cereal Disease Laboratory, United States Department of Agriculture-Agricultural Research Service, Saint Paul, Minnesota 55108.
Department of Plant Pathology, University of Minnesota, Saint Paul, Minnesota 55108.
G3 (Bethesda). 2017 Oct 5;7(10):3481-3490. doi: 10.1534/g3.117.300209.
Wheat stem rust, caused by f. sp. Eriks. & E. Henn, can incur yield losses in susceptible cultivars of durum wheat, ssp. (Desf.) Husnot. Although several durum cultivars possess the stem rust resistance gene , additional genes in durum wheat effective against emerging virulent races have not been described. Durum line 8155-B1 confers resistance against the f. sp. race TTKST, the variant race of the Ug99 race group with additional virulence to wheat stem rust resistance gene However, 8155-B1 does not confer resistance to the first-described race in the Ug99 race group: TTKSK. We mapped a single gene conferring resistance in 8155-B1 against race TTKST, , to chromosome arm 6AS by utilizing Rusty/8155-B1 and Rusty*2/8155-B1 populations and the 90K Infinium iSelect Custom bead chip supplemented by KASP assays. One marker, , cosegregated with in both populations and correctly predicted presence or absence in 11 durum cultivars tested. We confirmed the presence of in cultivar Mountrail by mapping in the population Choteau/Mountrail. The marker developed in this study could be used to predict the presence of resistance to race TTKST in uncharacterized durum breeding lines, and also to combine with resistance genes effective to Ug99 such as The map location of cannot rule out the possibility that this gene is an allele at the locus. However, race specificity indicates that is different from the known alleles and .
小麦秆锈病由禾柄锈菌小麦专化型（Puccinia graminis f. sp. tritici Eriks. & E. Henn）引起，可导致硬粒小麦（Triticum turgidum ssp. durum (Desf.) Husnot）的感病品种减产。尽管一些硬粒小麦品种携带秆锈病抗性基因Sr31，但尚未有针对新出现的毒性小种的硬粒小麦其他有效基因的报道。硬粒小麦品系8155 - B1对禾柄锈菌小种TTKST具有抗性，TTKST是Ug99小种组的变异小种，对小麦秆锈病抗性基因Sr31具有额外毒性。然而，8155 - B1对Ug99小种组中首个被描述的小种TTKSK不具有抗性。通过利用Rusty/8155 - B1和Rusty*2/8155 - B1群体以及90K Infinium iSelect定制芯片并辅以KASP分析，我们将8155 - B1中赋予对小种TTKST抗性的单个基因定位到了6AS染色体臂上。一个标记Kukri_c6_5444844与该基因在两个群体中均共分离，并正确预测了在11个测试硬粒小麦品种中该基因的存在与否。我们通过在Choteau/Mountrail群体中进行定位，证实了Mountrail品种中该基因的存在。本研究中开发的标记可用于预测未鉴定的硬粒小麦育种系中对小种TTKST抗性的存在情况，也可用于将该基因与对Ug99有效的抗性基因如Sr35进行聚合。该基因的图谱定位不能排除该基因是Sr31位点等位基因的可能性。然而，小种特异性表明该基因与已知等位基因Sr31和Sr36不同。
