Department of Hand and Microsurgery, Tianjin Hospital, Tianjin 300211, China.
Orthopaedics Integration of Traditional Chinese Medicine with Western Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
Biomed Res Int. 2017;2017:7238960. doi: 10.1155/2017/7238960. Epub 2017 Aug 10.
The authors aim to investigate protective effects of fasciotomy against ischemia reperfusion injury of skeletal muscle in rabbit and to compare the treatment effects of prereperfusion + fasciotomy and fasciotomy + postreperfusion against ischemia reperfusion injury of skeletal muscle. 24 healthy male Japanese white rabbits were randomly divided into 3 groups, and 4 hours' ischemia was established in these rabbits through surgery. Six hours' reperfusion was performed in group A; reperfusion + postfasciotomy was performed in group B; and prefasciotomy + reperfusion was performed in group C. Result showed that prefasciotomy and postfasciotomy could protect skeletal muscle against ischemia reperfusion injury, reduced MDA (malondialdehyde) expression, MPO (myeloperoxidase) expression, and apoptosis of muscle in the reperfused areas, increased Bcl-2 expression, and decreased Bax expression. The MDA and MPO levels in group B and group C were significantly lower than those in group A, and MDA and MPO levels in group C were significantly lower than those in group B. Prefasciotomy and postfasciotomy could protect against ischemia reperfusion injury in skeletal muscle. The protective effects of prefasciotomy against ischemia reperfusion injury are better than postfasciotomy.
作者旨在研究筋膜切开术对兔骨骼肌缺血再灌注损伤的保护作用，并比较预先切开筋膜+再灌注和再灌注后切开筋膜对骨骼肌缺血再灌注损伤的治疗效果。24 只健康雄性日本白兔随机分为 3 组，通过手术建立 4 小时的缺血。A 组进行 6 小时再灌注；B 组进行再灌注+筋膜切开术；C 组进行筋膜切开术+再灌注。结果表明，筋膜切开术可保护骨骼肌免受缺血再灌注损伤，减少 MDA（丙二醛）表达、MPO（髓过氧化物酶）表达和再灌注区肌肉凋亡，增加 Bcl-2 表达，减少 Bax 表达。B 组和 C 组的 MDA 和 MPO 水平明显低于 A 组，C 组的 MDA 和 MPO 水平明显低于 B 组。筋膜切开术可预防骨骼肌缺血再灌注损伤。筋膜切开术预先切开对缺血再灌注损伤的保护作用优于再灌注后切开。
