小麦条锈菌对小麦侵染力的评估
Assessment of Aggressiveness of Puccinia striiformis on Wheat.
作者信息
Sørensen Chris K, Thach Tine, Hovmøller Mogens S
机构信息
Department of Agroecology, Aarhus University, Forsøgsvej 1, 4200, Slagelse, Denmark.
出版信息
Methods Mol Biol. 2017;1659:41-47. doi: 10.1007/978-1-4939-7249-4_4.
A simple point-inoculation method using Novec™ 7100, a volatile engineered fluid, is presented for the assessment of aggressiveness of Puccinia striiformis isolates on seedlings of wheat. The method allows for quantification of the applied inoculum with a minimal risk of cross-contamination of rust from leaves grown side by side. The method is also applicable for the assessment of qualitative differences inferred by compatible and incompatible host-pathogen interactions, and it can be adjusted to other cereal rust and powdery mildew fungi on other host species, and other plant growth stages as appropriate.
本文介绍了一种使用挥发性工程流体Novec™ 7100的简单点接种方法，用于评估小麦条锈病菌株对小麦幼苗的侵染力。该方法能够对所接种的菌量进行定量，同时将并排生长叶片间锈病交叉污染的风险降至最低。该方法还适用于评估由亲和性和非亲和性寄主-病原体相互作用推断出的定性差异，并且可以根据需要调整，用于其他寄主物种上的其他谷物锈病和白粉病菌，以及其他植物生长阶段。
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